Black aspergilli are among the main causative agents of otomycosis worldwide. In this study, the species assignment of black aspergilli isolated from otomycosis cases in Iran was carried out using sequence analysis of part of the calmodulin gene. The results indicate that Aspergillus niger is not the only black Aspergillus species involved in otomycosis cases in Iran: Aspergillus awamori and Aspergillus tubingensis are also able to cause ear infections. Antifungal susceptibility tests were carried out against five antifungal drugs including amphotericin B, fluconazole, itraconazole, ketoconazole and terbinafine. All isolates were highly susceptible to terbinafine, while they exhibited moderate susceptibilities against amphotericin B, fluconazole and ketoconazole. Aspergillus niger and A. awamori were found to have higher minimal inhibitory concentrations for azoles than A. tubingensis, in accordance with previous findings.

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Soil organic matter is the largest source of organic carbon in the soil surface which plays an enormous role in restoring balance, environmental sustainability, soil elements and climatic conditions. Organic materials directly and indirectly influence physical, chemical and biological properties of soil and thus soil fertility. The amount, type and composition of organic matter are different in different soil and climatic conditions. Different soil components can hold different combination of soil organic matter. In this study soil samples were collected from virgin, Populus and Alnus forests from Guilan Province, Iran. The amount of organic matter in the original samples and sand, silt and clay fractions were measured and the quality of organic carbon (13 C) and hydrogen (1 H) was determined in fractions by Nuclear Magnetic Resonance. Based on the results, the original samples of Alnus forest showed the highest amount of organic carbon. Among fractions, clay showed the highest amount of organic carbon. The result of Nuclear Magnetic Resonance showed presence of different aliphatic and aromatic carbons and hydrogen in different samples. Virgin forest had the highest proportion of alkyl to oxygenated alkyl carbon ratio and Alnus forest showed the highest ratio of aliphatic to aromatic carbon. Both of above mentioned ratios was higher in clay fraction than two other fractions.