We used data collected on 48,739 cows from 1982 to 2013 from Foka farm in the Isfahan province of Iran to assess the reasons for culling in Iranian Holstein dairy cattle in the context of breeding values of economic traits, and also to study the relationship between longevity and production traits. Estimation of (co)variance components and genetic parameters for studied traits including MY, FY, herd life (HL) and length of production life (LPL) was by AI-REML algorithm in WOMBAT software. The results showed that 27.11% of cows were culled voluntarily under the farmers' command. The number and reasons for culling animals varied considerably over the years. Also, the results showed that involuntarily culled animals were valuable with significantly higher breeding values for MY and FY (P < .0001) than those of voluntarily culled individuals. The estimates of heritability for longevity traits (HL and LPL) were low (0.11 and 0.09), but their genetic correlation with production traits were high and positive (0.63-0.70). Our results suggest that some opportunity may exist for genetic improvements in fat and milk yield in Iranian Holsteins. ARTICLE HISTORY