Heavy metals are the stress factors that cause important problems in plant production and environment. Among the heavy metals that cause a decrease in plant growth and development, lead can have an important effect depending on its concentration. Higher concentrations in root rhizosphere can negatively impact plant growth and yield. In this study, parameters related to plant growth were investigated in beans grown in soil contaminated with lead at different concentrations (0, 1000, 1500, 2000 mg kg -1 ). The potting soil prepared in the greenhouse study was first treated with the indicated doses of lead and left to incubate for three weeks, and then seeds were sown in to the pots. In the study, the effect of lead application was evaluated on stem diameter, plant height, leaf area, leaf fresh weight, shoot fresh weight, root fresh weight, leaf dry weight, shoot dry weight, root dry weight, SPAD value, chlorophyll a, chlorophyll b, total chlorophyll, electrical leakage (EL) and leaf relative water content (LRWC) of bean seedlings. Findings of the study indicated that there was a significant decrease in growth and chlorophyll contents of bean seedlings in parallel with the increased lead dose. Also, with increasing lead level, LRWC decreased while EL increased. As a result, it has been determined that lead led to reduce in growth and development in bean seedlings, and seen that in the next stage, it will cause significant reductions in yield leads to economic loss of income for the producer.
Water stress causes significant problems in plant growth and development in arid and semi-arid regions in the world. Water stress symptoms especially appears during the seedling period of plants in many crops. However, the tolerance of plants to water stress can be increased with some exogenous biostimulant applications. The present study investigated the effect of exogenous biostimulant application on organic acid content of tomato seedlings under water stresses conditions. The study was conducted as pot experiment under controlled greenhouse conditions. Drought stress treatments was applied in two different levels; full irrigation (100%) and 50% of the field capacity in the study. 1% Zn, Bacillus subtilis, Bacillus megaterium, Azosprillum and Bacillus amyloliquefaciens (1x10 9 cfu/ml) mixture were used as a biostimulant treatment. The biostimulant solutions were prepared at a ratio of 1/10 and 1/5 and given to the tomato seedlings three times with one-week intervals as root drench. The effects of water stress and the solutions on organic acid content of tomato seedlings were determined in the study. The results were differed depending on the organic acid type under water stress and non-stress conditions. However, depending on the application doses; the negative effect of lower irrigation level on the organic acid content was alleviated. The results of the biostimulant application doses found statistically significant. In most of the organic acids, the application dose makes massive differences on the content of the organic acids. As a result, it is thought that the effect of lower irrigation level on tomato seedlings in terms of organic acid content can be improved by exogenous biostimulant applications.
In this study, the effect of biostimulant treatment against water stress in tomato seedling period was investigated. In the study conducted in the greenhouse conditions, water stress was applied in two different levels; full irrigation (100% of the field capacity) and 50% of the field capacity. The product used as a biostimulant has 1%Zn, Bacillus subtilis, Bacillus megaterium, Azosprillum and Bacillus amyloliquefaciens (1x10 9 cfu/ml) content.The solutions prepared at a ratio of 1/10 and 1/5 and were given to the plants three times with one-week intervals. The effects of water stress and biostimulant treatments on amino acid content in tomato seedlings were investigated. As a result of this study, some of the amino acid content in the plant leaf with has increased and some have decreased under different water availability conditions according to the amino acid type. However, depending on the application doses, it was seen that the negative effect of water stress on the amino acid content was alleviated. As a result of the current study, the effect of applications in reducing the damage caused by water stress on tomato seedlings may also be important in terms of amino acid content.
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