A mirror image phage display approach was used to identify novel and highly specific ligands for Alzheimer's disease amyloid peptide Abeta(1-42). A randomized 12-mer peptide library presented on M13 phages was screened for peptides with binding affinity for the mirror image of Abeta(1-42). After four rounds of selection and amplification the peptides were enriched with a dominating consensus sequence. The mirror image of the most representative peptide (D-pep) was shown to bind Abeta(1-42) with a dissociation constant in the submicromolar range. Furthermore, in brain tissue sections derived from patients that suffered from Alzheimer's disease, amyloid plaques and leptomeningeal vessels containing Abeta amyloid were stained specifically with a fluorescence-labeled derivative of D-pep. Fibrillar deposits derived from other amyloidosis were not labeled by D-pep. Possible applications of this novel and highly specific Abeta ligand in diagnosis and therapy of Alzheimer's disease are discussed.
Fünf Gesteinsmehle unterschiedlicher chemisch‐mineralogischer Zusammensetzung wurden bezüglich ihrer Eignung als Düngemittel in der Landwirtschaft untersucht.
Die Nährelementwirkung der Gesteinsmehle wird vor allem von der mineralogischen Zusammensetzung und dem Vermahlungsgrad bestimmt, jedoch nur in geringem Maße von den Nährelementgesamtgehalten.
Der geringe Gehalt verfügbarer Nährstoffe (unter 1%) und der hohe Anteil an für die Pflanzenernährung bedeutungslosen Stoffen (über 70% oxidisches Si, Al und Fe) stellen den Einsatz als Düngemittel, zumindest in annuellen Anbausystemen, in Frage.
Die Unterschiede zwischen den untersuchten Gesteinsmehlen sind so groß, daß der Begriff “Gesteinsmehl” für sich allein keine Aussagekraft besitzt.
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