An improved procedure employs a single-step extraction for determining naturally occurring vitamin A in chicken livers, synthetic vitamin A in fortified powdered formulas for infants, and stabilized vitamin A in animal feed supplements. Stabilized vitamin A is determined in the presence of , '-diphenyl-p-phenylenediamine by a simple modification of the procedure. The vitamin A is solubilized in the test material with the surfactant and extracted with a mixed solvent. Twenty-five minutes are required for one extraction and several may be run simultaneously. The procedure gives good recovery replication, and results comparable to those obtained by saponification. THE DETERMINATION OF VITAMIN A in food products usually requires saponification (2), which in the case of milk may take as long as 3 hours (3), as well as numerous
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