Shellard, Houghton, Resha Experimental and Results MaterialsYoung plants 24 months old (up to 1 m high) obtained by germination of seeds obtained from Bangkok and subsequently grown in the hot houses at Chelsea Physic Garden, the temperature being maintained 24-26' C and the relative humidity about 60°/o. MethodsThe method used was that described by SHELLARD and HOUGHTON [2] except that densitometry was not used for purposes of quantitation. Instead, personal observation of the coloured alkaloidal spots on TLC using 0.2 M FeC1, in 35 O/ o HClO, enabled an approximate comparative analysis of the amounts of the alkaloids present in various parts of the plant to be made. The score 10 was
h n t a = media ~b~t~e s e a r~h Abstract During the TLC examination of extracts of leaves and young twigs of Mitragyna speciosa from Thailand [I] there was evidence of small quantities of oxindole alkaloids corresponding to C (9)-OCH, heteroyohimbine alkaloids and of possible indole N-oxides. Some of them were isolated and in order to identify these alkaloids it was essential to have authentic alkaloids for comparative purposes, which could only be
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