Production of xylitol and ethanol, as well as activities of the key enzymes of D xylose consump tion, were studied in Pachysolen tannophilus mutants with altered growth on D xylose, xylitol, ethanol, or D glucose as sole carbon sources. Suppressed activity of xylose reductase with preferential affinity for NADPH and of xylitol dehydrogenase to 4.40 and 4.80 µmol mg -1 min -1 , respectively, resulted in accumu lation of xylitol (0.25 g per 1 g D xylose consumed). The highest levels of NADH/NADPH xylose reductase and xylitol dehydrogenase (6.00-6.80 and 6.80-8.40 µmol mg -1 min -1 , respectively) were found in the strains producing 0.24-0.26 g ethanol per 1 g D xylose. Application of Pa. tannophilus mutants for analysis of the regulation of D xylose catabolism in yeasts is discussed.
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