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An experiment was carried out to study the genetic divergence of 39 genotypes of blackgram. Biochemical parameters such crude protein, albumin and globulin content and yield attributing traits such as hundred seed weight and single plant yield were estimated. Grouping of genotypes into various clusters was done by Mahalnobis D 2 statistics. The 39 genotypes were grouped into five clusters. Among the five clusters, cluster V had more number of genotypes. The highest value of intra cluster distance was recorded in cluster V while cluster I had minimum intra cluster distance and maximum inter cluster distance was observed between cluster II and cluster III. Among the nine characters single plant yield and hundred seed weight contributed maximum towards divergence. The study showed that the genotypes UH04-4, CO 5, KKB05011 and ACM07002 could be used for hybridization to develop high yielding blackgram varieties with good battering quality.

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