MATERIALS AND METHODS
MaterialsThe plant material used was taken from Ciamis Regency, West Java Province, Indonesia. Crops were harvested on March 24, 2021 and then determined. Voucher specimens are stored at the Bandungense Herbarium, Bandung Institute of Technology, with registration numbers: Zingiber officinale Roscoe namely FIPIA-DEP30, Zingiber montanum (J.Koenig) Link ex A., namely FIPIA-DEP29 and Zingiber zerumbet (L.) Roscoe ex Sm. , namely FIPIA-DEP27. The bacteria used were Propionibacterium acne ATCC 11827, the culture medium was Nutrien Agar (Oxoid), and dimethyl sulfoxide /DMSO 99,9 % (Merck) as extract solvent. The materials used in the extraction process are ethanol 70%, the materials used for fractionation are methanol, ethylacetate 99.8% (Satyam), n-hexane 99.9% (Alfa), and aquadestylates (Brataco). Materials used for TLC: Silica Gel 60 F254 (Merck) was used in stationary phase with methanol (Merck) as mobile phase, and
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.