Abstract—
The accumulation of phytochrome in the dark was measured for Avena sativa seedlings after a white light pretreatment and for Sorghum vulgare seedlings after continuous red or far‐red light treatments, using the herbicide Norflurazon to prevent greening under continuous irradiation. In both cases the accumulation of phytochrome depends on the state of the phytochrome at the light‐dark transition: high Pfr levels (red light pulse) led to a slower rate of phytochrome accumulation than lower Pfr levels (long wavelength far‐red (RG 9) light pulse). Poly‐(A+)‐RNA was isolated fromA. sativa seedlings grown for 48 h in darkness + 24 h WL + light pulse (5 min) (red, RG 9 light, red followed by RG 9 light or RG 9 followed by red light pulse) + 19 h darkness. The poly‐(A+)‐RNA was translated in a rabbit reticulocyte lysate system and the translation products were immunoprecipitated by specific anti‐phytochrome antibodies. It was demonstrated that the activity of mRNA coding for phytochrome was under phytochrome control.
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