Extracts of the leaves of Chrysanthemum parthenium (feverfew) inhibit the secretory activity of human blood platelets and polymorphonuclear leukocytes (PMNL) and it has been suggested that the inhibitory effect on platelets is mediated by a neutralization of cellular sulphydryl groups. The results indicate that the effect of feverfew) on PMNL behaviour may be via blockade of sulphydryl groups in the cells.Venous blood from healthy volunteers was collected into 1/10 volume of 0.1 M trisodium citrate dihydrate as anti-coagulant. The blood was centrifuged for 15 minutes at 250 g and the supernatant plasma was discarded. To each 1 ml of the cell sediment, 2 ml of PBS [that contained 5.5 mM glucose, 0.5% (w/v) bovine serum albumin (BSA), and 0.01 mM trisodium citrate dihydrate], 1 ml of 6% (wtv) dextrane solution (Infukoll 70, Arzneimittelwerk Dresden, GDR) and 3 U heparin were Downloaded by: Universite Laval. Copyrighted material.

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