summaryThe mechanisms and kinetic course of BOD exertion were compared in both open and closed systems. Two open reactors, a simulated stream device, and an open stirred reactor were employed, and the closed systems consisted of standard BOD bottles and 2.4-liter vessels. In the closed systems, both quiescent and stirred conditions of incubation were examined. Biological solid? concentration, bacteria and protozoa concentration, substrate analysis, and chemical oxygen demand as well as biochemical oxygen utilization were employed to assess the performance of these systems.Oxygen uptake rate constants were observed to increase with increasing concentration of carbon source, thus militating against direct use of the usual dilution technique for predicting rate of deoxygenation in receiving streams. The relationship between specific O2 uptake rate and substrate concentration approximated a hyperbolic function similar to the Blonod relationship for specific growth rate and substrate concentration. A technique using an open stirred reactor rather than the standard BOD bottle dilution technique is recommended.
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