The Asian Seabass farming can be successful with optimum environmental conditions. Water quality as a culture media must always be maintained to suppress the occurrence of pathogen attacks on fish. The study aims to determine the effect of temperature, pH, and salinity in the maintenance of body weight Asian Seabass (Lates calcarifer) at different stocking. The field experiment method was applied in this study using a 6.28 m3 tank using 2 treatments, namely P1 (density 1000) and P2 (density 1400) fish measuring 2-3 cm for 30 days. Parameters observed included observations of growth and water quality (temperature, pH, and salinity). The result showed that there was an effect of temperature, pH, and salinity on the growth of Asian Seabass. The temperature, pH, and salinity showed an effect on body weight by P1 (35.2%) and P2 (28.9%). The conclusion of this study is that the density of 1000 shows a higher effect that the density of 1400 on the growth of Asian Seabass.