Two field experiments were conducted at Rice Research and Training Center ( RRTC ) Farm, Kafr-El-Sheikh Governorate, Egypt, during two successive seasons of 2009 and 2010. The main objectives of this study are; identifying both the optimum seeding rates and hill spacing to increase the yield of hybrid rice seed for the hybrid IR69625A/Giza181R (2046H), this study included, three seeding rates for cytoplasmic male sterile line (6kg/fed, 8kg/fed and 10kg/fed) and three hill spacing ( 15×10, 15×15 and 15× 20cm).The treatments were arranged in split plot design with three replications, where seeding rates distributed as main plots while hill spacing were allocated in the sub plots. Data were recorded on different traits relating to yield and its components .The results showed that, the seed rates had highest significant on plant height , panicle weight, panicle length, panicle exsertion, no. of spikelets/ panicle seed set, 1000 grain weight and grain yield . The optimum treatment to produce high quantity of hybrid rice seed from the hybrid combination of IR69625A/Giza181R (SK.2046H) was 8kg seeds/fed, for the seeding rate.Moreover, hill spacing treatments had highest significant on all studied characters,except no. of panicles/m 2 character. The best hill spacing for cytoplamic male sterile was 15 x 15cm.The interaction between seed rates and hill spacing was significant for all studied characters.It could be concluded that the optimum treatments to produce high quantity of hybrid seed from the hybrid combination IR69625A/Giza 181R (SK.2046H) was 8kg seeds/fed for the seeding rate and (15×15cm) for hill spacing, because this treatment was recorded the highest values of panicle exsertion (81.61%),seed set (46.06%) and grain yield (1.32 t/fed).
Recently, salt tolerance protein (STO) of Arabidopsis and rice were identified and confirmed by STO in yeast cell. Here, we present data of one STO family in Arabidopsis which protein was cloned, and the expression levels of the genes were analysed by Reverse transcriptase (RT)-PCR, using gene-specific primers. We examined the expression of AtSTO mRNA under different stress conditions. The whole plants in age two weeks were treated by (1mM H2O2), NaCl 0, 100, 200, 400 mM of NaCl concentration and 20% for PEG and were incubated for different time. For Brefeldin A (BFA) treatments, were added to the Arabidopsis cells culture in three days growth at concentration of 10 mg/ml, and the samples were collected at different times from added BFA. we present data of one STO family in Arabidopsis which has Zinc-finger protein, and our result indicted that H2O2, BFA were inhibitor of the activity of AtSTO mRNA and H2O2 decrease the expression level of AtSTO mRNA. Generally the expression of STO in presence of different concentrations of NaCl and other stresses factors showed that STO protein play a major role to drought tolerance.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.