The period in which antimouse kidney serum (AKS) was embryolethal in the mouse extended from days 4-15 of pregnancy. The highest sensitivity toward AKS existed at days 10-14.When AKS was localized in the uterus by immunofluorescence the following results were obtained. On day 10 Reichert's membrane, parietal yolk-sac cells, and endodermal cells of the visceral yolk sac, but not the embryo, showed intense fluorescence. By day 12 Reichert's membrane and the parietal yolk-sac cells had lost most of their immunofluorescence. The visceral yolk-sac cells adjacent to the chorioallantoic placenta still stained strongly on day 18 of pregnancy.AKS from which the antiplacental factor(s) were removed retained its embryolethality when injected on day 10. It was localized solely i n the visceral yolk sac.Therefore AKS probably caused embryonic death by damaging the yolk-sac cells.Only during the last days of pregnancy was there any transfer of AKS to the embryos. of staining may then be caused by a reduced structural integrity. Anderson, J. W. 1959 The placental barrier toNo exact explanation C a n be given at gamma-globulin in the rat. Am. J. Anat., 104: present for the lack of immunofluorescence 403430. LITERATURE CITED~~ if uteri from mice injected with normal Baart de la Faille-Kuyper, E. H. 1969 Lupus rabbit Serum (NRS). However, it is likely erythematosus. A clinical and immunohistochemical study of 485 patients. M.D. thesis. that the visceral endodermal cells retain Schotanus and Jens, Utrecht.
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