Summary
Isolated protoplasts of 60 species of the Magnoliatae and 19 species of the Liliatae were embedded in droplets of agarose‐containing media in such a way that streaks of locally high cell densities were obtained. For comparison, agarose cultures with dispersed protoplasts and liquid cultures were prepared. Fast and reliable regeneration was obtained in several taxa. After transfer to low osmolar media, shoots were formed in 18 species belonging to the genera Arabidopsis, Asparagus, Biscutella, Brassica, Cichorium, Clianthus, Hypericum, Nicotiana, Petunia, Reseda, Senecio, and Solanum. Only protoplast‐derived callus which did not organize shoots was grown in species of Agrimonia, Daucus, Cucumis, Duchesnea, Helianthus, Potentilla, Prunus, Reseda, Solanum, Sorbus, Spinacia, and Vicia.
Organized development from isolated protoplasts using a single protocol has been obtained with two bryophyte species, one fern species, and 34 dicotyledonous species. Regeneration is described here for the first time with the dicotyledonous species Cyphomandra betacea, Euphorbia pulcherrima, Linum catharticum, and Senecio fuchsii. In co‐cultures with a Solanum hybrid, interfamilial and even interdivisional support was observed acting at different developmental steps. The results are discussed with respect to standardization of culture conditions, variety of morphogenic pathways, and interspecific correlations such as tolerance, allelopathy and nursery/feeding in unrelated systems.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.