Abstract. Intracytoplasmic hyaline globules were found frequently in chromaffin cells of the adrenal medulla from several species of laboratory animals that died from various causes. The globules stained intensely red with PAS, were weakly acid-fast, did not stain with oil-red 0 and showed bright yellow autofluorescence. Ultrastructurally , they were finely granular, round to oval, intensely osmiophilic and were surrounded by an intimately bound trilaminar membrane. The globules were interpreted as aggregated secretory granules that occur in degenerating cells.Intracytoplasmic hyaline globules in the adrenal medulla have been recognized in several animal species [ 5 , 91 including nonhuman primates [14]. They were first described in 1909 in people that died of infectious disease. These globules were interpreted as phagocytized erythrocytes [16]. Since then they have been found associated with various conditions including infectious disease, neoplasia and poisoning. Materials and MethodsThe adrenal glands from mice, rats, hamsters, guinea pigs, rabbits, dogs, opossums and Rhesus monkeys used in dibenzo-p-dioxin and chlorinated biphenyl toxicologic studies and from animals that died from spontaneous disease (mainly bacterial) were examined for intracytoplasmic hyaline globules in the chromaffin cells of the medulla. All tissues for light microscopy were collected soon after death and were fixed in 10 percent neutral buffered formalin. Sections were routinely stained with hematoxylin and eosin (HE). Special stains were periodic acid-Schiff (PAS), oil-red 0, Ziehl-Neelsen acid-fast and Millon's reaction for tyrosine [19].Parts of adrenal medulla for electron microscopy were removed from guinea pigs while the animals were anesthetized. Tissues were fixed in 2.5 percent glutaraldehyde and 2.0 percent paraformaldehyde in cacodylate buffer (pH 7.4) for 6 hours, washed with cacodylate buffer (pH 7.4), then put in 1 percent osmium tetroxide in cacodylate buffer (pH 7.4) for 2 hours, dehydrated in alcohol and propylene oxide, and embedded in epon 812. Thick sections (0.5 micrometers) for light microscopy were cut with an ultramicrotome and stained with toluidene blue. Thin sections, 400-600 x lo-' millimeters by interference color, were stained with uranyl acetate and Reynold's lead citrate [17]. 435
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