Aim We focus on the biogeographical role of the Balkan Peninsula as a glacial refugium and source of northward post‐glacial dispersal for many European taxa. Specifically, we analysed the genetic structure and variation of wild boar (Sus scrofa) samples primarily from Greece, a region that has repeatedly served as a glacial refugium within the Balkan Peninsula. Location Continental Greece, the Aegean island of Samos and Bulgaria. Methods We analysed wild boar samples from 18 localities. Samples from common domestic breeds were also examined to take into account interactions between wild and domesticated animals. Phylogenetic analyses were carried out on a 637‐bp fragment of the mitochondrial DNA control region in 200 wild boar and 27 domestic pigs. The sequences were also compared with 791 Eurasian wild boar and domestic pig D‐loop sequences obtained from GenBank. Results Ninety‐four haplotypes were identified in the European wild boar data set, of which 68 were found in the Balkan samples and assigned to two previously described clades: the E1 European and Near Eastern clades. All of the continental samples clustered in the E1 clade and the samples from Samos fell into the Near Eastern clade, consistent with the island’s proximity to Asia Minor. Intriguingly, 62 novel haplotypes were identified and are found exclusively in the Balkans. Only six haplotypes were shared between wild boar and domestic pigs. Main conclusions Our data reveal numerous novel and geographically restricted haplotypes in wild boar populations, suggesting the presence of separate refugia in the Balkans. Our analyses support the hypothesis of a post‐glacial wild boar expansion consistent with the leading edge model, north and west from modern day Greece, and suggest little maternal introgression of Near Eastern and domestic haplotypes into wild Balkan populations.
Forested areas provide important breeding habitats for the turtledove (Streptopelia turtur) in DadiaLefkimi-Soufli National Park, Northeastern Greece. We censused the birds in two forested habitat types using the point-count technique at 60 sites during the breeding season (from mid-April to mid-June) in 2001 and 2002. We sampled vegetation structure at the same sites by measuring horizontal (tree species and density in different size classes) and vertical (percentage canopy closure in dominant, intermediate, suppressed and shrub layer) characteristics within 0.04 ha circular plots centred on the established points. Univariate and multivariate statistical techniques were employed to examine the response of the turtledove′s presence to habitat differences between used and unused sites. The results of this study indicate that habitat structure influence the presence of the turtledove during the breeding season. Middle-aged forest stands particularly those dominated by pine trees with low percentage cover in understory are likely to be beneficial to breeding the turtledove population. A combination of multipurpose forestry operations allowing development of managed woodland in mosaics with other habitat types could provide high-quality habitats for a wide range of wildlife species including game and non-game species in the area. Silvicultural methods of maintaining appropriate breeding habitat for turtledove in Dadia-Lefkimi-Soufli National Park (DLS NP), which are in conflict with commercial forestry, are discussed.
Stone martens (Martes foina) are documented as generalist throughout their distributional range whose diet composition is affected by food availability. We tested if this occurs and what feeding strategies it follows in a typical Mediterranean ecosystem in Central Greece by analysing contents from 106 stomachs, seasonally collected from three different habitats during 2003–2006. Seasonal variation in diet and feeding strategies was evident and linked to seasonal nutritional requirements, but possibly imposed by strong interference competition and intraguild predation. Fleshy fruits and arthropods predominated in the diet, but also mammals and birds were frequently consumed. An overall low dietary niche breadth (B A = 0.128) indicated a fruit specialization tendency. A generalised diet occurred in spring with high individual specialisation, whereas more animal-type prey was consumed than fruits. A population specialization towards fruits was indicated during summer and autumn, whereas insects were consumed occasionally by males. In those seasons it switched to more clumped food types such as fruits and insects. In winter it selectively exploited both adult and larvae insects and partially fruits overwinter on plants. The tendency to consume particular prey items seasonally reflected both the population specialist behaviour and the individual flexibility preyed on different food resources.
Aim We compared the power of different nuclear markers to investigate genetic structure of southern Balkan wild boar. We distinguished between historic events, such as isolation in different refugia during glacial periods, from recent demographic processes, such as naturally occurring expansions.Location Southern Balkans/Greece. MethodsWe sampled 555 wild boars from 20 different locations in southern Balkans/Greece. All individuals were analysed with 10 microsatellites and a subgroup of 91 with 49,508 single nucleotide polymorphisms (SNPs). Patterns of genetic structure and demographic processes were assessed with Bayesian clustering, linkage disequilibrium and past effective population size estimation analysis.Results Both microsatellite and SNP data analyses detected genetic structure caused by historic events and support the existence of three groups in the studied area. A hybrid zone between two of the groups was also detected. We also showed that genome-wide SNP data analysis can identify recent events in bottlenecked populations. Main conclusionsWe inferred the three groups diverged~50,000-10,000yr bp when populations contracted to different refugia. Our findings strengthened the evidence that the southern Balkan area was a glacial refugium including further local smaller refugia. Genome-wide genotyping inferred a recent population expansion that can mimic a 'refugium within refugium' scenario. It seems that microsatellite data tend to overestimate genetic structure when genetic drift happens in bottlenecked populations over a short distance. Therefore, genome-wide SNPs are more powerful at inferring phylogeography in natural populations, resolving inconsistencies from mitochondrial and microsatellite data sets.
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