The aim of the present study was to analyze the variation of esterase activity as well as the expression patterns of isozymes by isoelectric focusing on polyacrylamide gels (IF-PAGE) and conventional polyacrylamide gel electrophoresis (PAGE). Esterase activity and isoforms were analyzed on total proteins from 9 isolates of Beauveria bassiana, from different hosts and geographic areas from the State of Guerrero, Mexico. Total proteins were separated by IF-PAGE and PAGE, in order to characterize the activity and isoelectric points of the esterase isoforms detected on different isolates. Electrophoretic analysis from 9 isolates of Beauveria bassiana, revealed a polymorphic pattern in terms of esterases activity and the presence of isozymes, showing a total of 10 different isoforms with variations in both the activity and isoelectric point. Each isolate showed between 3-6 different isozymes. Cluster analysis derived from the Jaccardś coefficients showed a significant association between the presence-absence of esterase isoforms and the geographical origin of the Beauveria bassiana isolates. We conclude that the use of this methodology for functional detection combined with PAGE and IF-PAGE, can be an excellent tool for biochemical characterization of isolates and further impact evaluation of artificial epizootics derived from Biological control programs.
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