Objectives: (1) To compare PCR method using ureA gene-specific primers and rapid urease test (RUT) for the diagnosis of H. pylori infection in gastric biopsy specimens; and (2) to determine the prevalence of H. pylori infection among patients with gastroduodenal diseases by the combination of both methods. Materials and method: Gastric biopsy specimens were collected from by endoscopy from 106 patients with gastroduodenal diseases. H. pylori infection was determined by the rapid urease test (RUT), followed by the PCR using ureA gene-specific primers. Results: This study reveals a high-level concordance (κ = 0.885; 95%CI: 0.796 – 0.974) between PCR and RUT for the diagnosis of H. pylori infection. However, PCR detected H. pylori in 5 (10.4%) of RUT-negative patients; and only 1 (1.7%) of RUT-positive cases were PCR-negative. The prevalence of H. pylori infection diagnosed by both PCR and RUT methods was 53.7%. The H. pylori infection was prevalent in gastroduodenal ulcers and patients with unknown medical history, 75% (p = 0.015) and 63.5% (p = 0.029), respectively. Conclusion: PCR using ureA gene-specific primers can detect several cases with H. pylori infection overlooked by RUT. The prevalence of H. pylori infection was still high, particularly in gastroduodenal ulcers and patients with an unknown medical history. Key words: rapid urease test (RUT), H. pylori, ureA gene-specific primers
Background: We examined the clinicopathologic features and carcinoembryonic antigen (CEA) levels of the colorectal carcinoma patients at Hue University Hospital and Hue Central Hospital. The aim of this study was to evaluate the relation between the pre-operative CEA levels from the post-operative CEA level and the clinicopathologic features in colorectal carcinomas. Materials and methods: From May 2011 to April 2012, samples were collected from 87 colorectal carcinoma patients. The pre-operative and post-operative samples from peripheral blood of the patients were collected to quantity of CEA level by sandwich principle. Results: Most patients were among the age of ≥70; 36.8% of cases. The medium age at presentation was 61.2±15,2. 36.8% (n=32) of the carcinomas were located in rectum and 19.5% in sigmoid colon. The great majority of cases were non-mucinous adenocarcinoma (90.8%). 48,1% of cases with pre-operative CEA level were higher normal level. The medium level of pre-operative CEA was 11.2±16.8 ng/l. Dukes B, C and D classification were 85.1%; 5.7% and 9.2% of cases, respectively. Compared with the pre-operative CEA level, post-operative CEA level was reduced (r = 0.604; p = 0.002). Conclusions: We found that there was a significant relation between pre-operative CEA level from post-operative CEA level (p<0.05), tumor location (p<0.05) and Dukes classification (p<0.05). Key words: CEA, colorectal carcinoma, clinicopathology
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.