Background: Previous studies have investigated technology-aided needling training systems for acupuncture on phantom models using various measurement techniques. In this study, we developed and validated a vision-based needling training system (noncontact measurement) and compared its training effectiveness with that of the traditional training method. Methods: Needle displacements during manipulation were analyzed using OpenCV to derive three parameters, i.e., needle insertion speed, needle insertion angle (needle tip direction), and needle insertion length. The system was validated in a laboratory setting and a needling training course. The performances of the novices (students) before and after training were compared with the experts. The technology-aided training method was also compared with the traditional training method. Results: Before the training, a significant difference in needle insertion speed was found between experts and novices. After the training, the novices approached the speed of the experts. Both training methods could improve the insertion speed of the novices after 10 training sessions. However, the technology-aided training group already showed improvement after five training sessions. Students and teachers showed positive attitudes toward the system. Conclusion:The results suggest that the technology-aided method using computer vision has similar training effectiveness to the traditional one and can potentially be used to speed up needling training.