The intrinsically photosensitive retinal ganglion cells (ipRGCs) provide a conduit through which rods and cones can access brain circuits mediating circadian entrainment, pupillary constriction and other non-image-forming visual functions. We characterized synaptic inputs to ipRGCs in rats using whole-cell and multielectrode array recording techniques. In constant darkness all ipRGCs received spontaneous excitatory and inhibitory synaptic inputs. Light stimulation evoked in all ipRGCs both synaptically driven ('extrinsic') and autonomous melanopsin-based ('intrinsic') responses. The extrinsic light responses were depolarizing, about 5 log units more sensitive than the intrinsic light response, and transient near threshold but sustained to brighter light. Pharmacological data showed that ON bipolar cells and amacrine cells make the most prominent direct contributions to these extrinsic light responses, whereas OFF bipolar cells make a very weak contribution. The spatial extent of the synaptically driven light responses was comparable to that of the intrinsic photoresponse, suggesting that synaptic contacts are made onto the entire dendritic field of the ipRGCs. These synaptic influences increase the sensitivity of ipRGCs to light, and also extend their temporal bandpass to higher frequencies. These extrinsic ipRGC light responses can explain some of the previously reported properties of circadian photoentrainment and other non-image-forming visual behaviours.

Intrinsically photosensitive retinal ganglion cells (ipRGCs) are photoreceptors of the mammalian eye that drive pupillary responses, synchronization of circadian rhythms, and other reflexive responses to daylight. Melanopsin is the ipRGC photopigment, but the signaling cascade through which this invertebrate-like opsin triggers the photocurrent in these cells is unknown. Here, using patch-clamp recordings from dissociated ipRGCs in culture, we show that a membrane-associated phosphoinositide cascade lies at the heart of the ipRGC phototransduction mechanism, similar to the cascade in rhabdomeric photoreceptors of invertebrate eyes. When ipRGCs were illuminated, melanopsin activated a G protein of the G(q/11) class, stimulating the effector enzyme phospholipase C. The presence of these signaling components in ipRGCs was confirmed by single-cell RT-PCR and immunofluorescence. The photoresponse was fully functional in excised inside-out patches of ipRGC membrane, indicating that all core signaling components are within or tightly coupled to the plasma membrane. The striking similarity of phototransduction in ipRGCs and invertebrate rhabdomeric photoreceptors reinforces the emerging view that these cells have a common evolutionary origin.

Horizontal, bipolar, and amacrine cells in the zebrafish retina were morphologically characterized using DiOlistic techniques. In this method, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI)-coated microcarriers are shot at high speed onto the surfaces of living retinal slices where the DiI then delineates axons, somata, and dendrites of isolated neurons. Zebrafish retinal somata were 5-10 microm in diameter. Three horizontal cell types (HA-1, HA-2, and HB) were identified; dendritic tree diameters averaged 25-40 microm. HA somata were round. Cells classified as HA-2 were larger than HA-1 cells and possessed an axon. HB somata were flattened, without an axon, although short fusiform structure(s) projected from the soma. Bipolar cells were separated into 17 morphological types. Dendritic trees ranged from 10 to 70 microM. There were six B(on) types with axon boutons only in the ON sublamina of the inner plexiform layer (IPL), and seven B(off) types with axon boutons or branches only in the OFF sublamina. Four types of bistratified bipolar cells displayed boutons in both ON and OFF layers. Amacrine cells occurred in seven types. A(off) cells (three types) were monostratified and ramified in the IPL OFF sublamina. Dendritic fields were 60-150 microM. A(on) pyriform cells (three types) branched in the ON sublamina. Dendritic fields were 50-170 microM. A(diffuse) cells articulated processes in all IPL strata. Dendritic fields were 15-90 microM. These findings are important for studies examining signal processing in zebrafish retina and for understanding changes in function resulting from mutations and perturbations of retinal organization.

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Studies in visual, auditory, and somatosensory cortices have revealed that different cell types as well as neurons located in different laminae display distinct stimulus response profiles. The extent to which these layer and cell type-specific distinctions generalize to gustatory cortex (GC) remains unknown. In this study, we performed extracellular recordings in adult female mice to monitor the activity of putative pyramidal and inhibitory neurons located in deep and superficial layers of GC. Awake, head-restrained mice were trained to lick different tastants (sucrose, salt, citric acid, quinine, and water) from a lick spout. We found that deep layer neurons show higher baseline firing rates (FRs) in GC with deep-layer inhibitory neurons displaying highest FRs at baseline and following the stimulus. GC's activity shows robust modulations before animals' contact with tastants, and this phenomenon is most prevalent in deep-layer inhibitory neurons. Furthermore, we show that licking activity strongly shapes the spiking pattern of GC pyramidal neurons, eliciting phase-locked spiking across trials and tastants. We demonstrate that there is a greater percentage of taste-coding neurons in deep versus superficial layers with chemosensitive neurons across all categories showing similar breadth of tuning, but different decoding performance. Lastly, we provide evidence for functional convergence in GC, with neurons that can show prestimulus activity, licking-related rhythmicity and taste responses. Overall, our results demonstrate that baseline and stimulus-evoked firing profiles of GC neurons and their processing schemes change as a function of cortical layer and cell type in awake mice.

Animals actively acquire sensory information from the outside world, with rodents sniffing to smell and whisking to feel. Licking, a rapid motor sequence used for gustation, serves as the primary means of controlling stimulus access to taste receptors in the mouth. Using a novel taste-quality discrimination task in head-restrained mice, we measured and compared reaction times to four basic taste qualities (salt, sour, sweet, and bitter) and found that certain taste qualities are perceived inherently faster than others, driven by the precise biomechanics of licking and functional organization of the peripheral gustatory system. The minimum time required for accurate perception was strongly dependent on taste quality, ranging from the sensory-motor limits of a single lick (salt, ϳ100 ms) to several sampling cycles (bitter, Ͼ500 ms). Further, disruption of sensory input from the anterior tongue significantly impaired the speed of perception of some taste qualities, with little effect on others. Overall, our results show that active sensing may play an important role in shaping the timing of taste-quality representations and perception in the gustatory system. IntroductionAnimals acquire information about their environment through active sensing. Rodents use rapid stereotyped behaviors such as sniffing and whisking to sample olfactory and tactile stimuli, with neural activity in these systems precisely aligned to the cycles of sampling behavior (Hill et al., 2011;Shusterman et al., 2011; Wachowiak, 2011). In the gustatory system, taste stimuli are sensed through the active process of licking, a rapid and stereotyped behavior that is the gustatory analog of sniffing in olfaction (Travers et al., 1997). During licking, taste stimuli are actively pulled into the mouth by the animal, creating a natural and sequential flow of information beginning from the tip of the tongue and following throughout the oral cavity (Reis et al., 2010). Although a prerequisite for tasting, the role of licking in shaping sensory processing in the gustatory system is poorly understood due in part to the use of a variety of experimental methods for delivering liquid taste stimuli that circumvent or alter the natural sequence of events associated with licking and active sensing (Katz et al., 2002b;MacDonald et al., 2009). Injection of liquid stimuli into the mouth of alert animals via intra-oral cannulas (IOCs) or pressurized lick spouts provides a rapid and reliable method of stimulus delivery for studying taste coding and perception. However, pressurized lick spouts and IOCs add a degree of passivity into the active process of tasting, potentially obscuring important aspects of gustatory sensory processing. Unlike other sensory systems that transmit information from the receptor organ to the brain through a single nerve, neural information about taste is brought into the brain by three separate nerves with anatomically and functionally distinct receptive fields (Shingai and Beidler, 1985; Spector and Travers, 2005; Spector and Glendinning,...

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The suprachiasmatic nucleus (SCN), the mammalian circadian pacemaker, receives information about ambient light levels through the retinohypothalamic tract. This information resets the molecular clock of SCN neurons, thereby entraining overt animal behavior and physiology to the solar cycle. Progress toward functional characterization of retinal influences on the SCN has been hampered by limitations of established experimental paradigms. To overcome this hurdle, the authors have developed a novel in vitro preparation of the rat retinohypothalamic circuit that maintains functional connectivity between the retinas and the SCN. This method permits whole-cell patch-clamp recordings from visually identified, light-responsive SCN neurons. Using this preparation, the authors have found that in the SCN, light-evoked responses are partly driven by the melanopsin photosensory system of the intrinsically photosensitive retinal ganglion cells and that SCN neurons exhibit light adaptation. The authors have also been able to generate this preparation from mice, demonstrating the feasibility of applying this method to transgenic mice.