Safflower (Carthamus tinctorius L.) is a multipurpose oilseed crop. Fusarium wilt (Fusarium oxysporum f.sp. carthami) is the major damaging disease in safflower. In the present investigation, Fusarium wilt resistance was introgressed from two wild species, Carthamus oxyacantha and Carthamus palaestinus, into susceptible cultivated species through interspecific hybridization. Inheritance of wilt resistance indicated single dominant gene control. Eight simple-sequence-repeat (SSR) markers each in ('Nira' × C. oxyacantha) and ('Nira' × C. palaestinus) were found to be linked to wilt resistance. Marker-assisted selection for wilt resistance was performed using these markers in F 3 -F 7 generations of both crosses. Six wilt resistant interspecific lines evaluated for 2 years under nondisease conditions have recorded 9%-29% higher seed yield than the high yielding cultivar, 'A1'. The wilt resistant lines would serve as new sources of resistance to wilt in safflower. The SSR markers linked to wilt resistance would be useful for precise selection of wilt resistance at seedling stage in large segregating populations without attempting screening in artificially inoculated conditions and pyramiding of wilt resistant genes from wild into a common background.
Several species of the soil borne fungus of the genus Trichoderma are known to be versatile, opportunistic plant symbionts, and are the most successful biocontrol agents used in today's agriculture. To be successful in the field conditions, the fungus must endure varying climatic conditions. Studies have indicated that high atmospheric temperature coupled with low humidity is a major limitation for the inconsistent performance of Trichoderma under field conditions. Understanding the molecular modulation associated with such Trichoderma that persist and deliver under abiotic stress condition will aid in exploiting the worth of these organisms for such use. In this study, comparative proteomic analysis using two dimensional gel electrophoresis (2DE) and matrix assisted laser desorption/time of flight (MALDI-TOF-TOF) mass spectrometry was used to identify proteins associated with thermotolerance in two thermotolerant isolates of Trichoderma: T. longibrachiatum 673, TaDOR673 and T. asperellum 7316, TaDOR7316 and 32 differentially expressed proteins were identified. Sequence homology and conserved domains were used to identify these proteins and to assign probable function to them. Thermotolerant isolate, TaDOR673, seemed to employ the stress signaling MAPK pathways and heat shock response pathways to combat the stress condition whereas the moderately tolerant isolate, TaDOR7316, seemed to adapt to high temperature conditions by reducing the accumulation of misfolded proteins through unfolded protein response pathway and autophagy. Also, there were unique as well as common proteins that were differentially expressed in the two isolates studied.
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