PURPOSE:To evaluate the effect of a local application of simvastatin gel in repairing bone defects in the femurs of rabbits.
METHODS:Two standard surgical cavities were created in the femoral epiphysis of 18 rabbits. In the simvastatin group (SG), the cavities were filled with a collagen sponge soaked in 0.5 ml of a simvastatin (1 mg) gel, and the cavities were covered with a biological membrane. The bone cavities in the second group (control group) were filled with a blood clot and covered with a biological membrane.On the 7 th, 21st and 42 nd days, six animals in each group were euthanized, and the femurs were subject to histological evaluation (vascularity, fibrosis, reactive bone formation, osteoblasts, and osteoclasts) and immunohistochemical (anti-VEGF and anti-osteocalcin) analysis.The results were analyzed using a Wilcoxon test (p<0.05).
RESULTS:There were significant differences between the two groups: the SG had greater scores in comparison with the CG in terms of the degree of vascularity on the 7t h and the 21st days, fibrosis on the 21 st day, bone formation reaction on the 21 st and the 42 nd days and the number of osteoblasts and osteoclasts on the 42 nd day. The immunohistochemical expression was also greater for osteocalcin and VEGF on the 7 th, 21st and 42 nd days.
CONCLUSION:Surgical defects created in rabbit femurs were treated locally with simvastatin gel to stimulate bone repair, which promoted an ameliorative effect in the morphological and immunohistochemical markers of bone regeneration.
A 24-year follow-up with simultaneous treatment of the lip, nose, and palate in the neonatal period is presented. The first author operated on 92 patients in the neonatal period using a personal approach based on the Rose and Spina techniques. Technical details of the primary repair are described. The unilateral cleft lip-nose is operated on through a curved skin incision, with an upper third Z-plasty. Extensive detachment of the musculature is realized without incising the sulcus. Two flaps close the mucosal layer in an S-shaped position. The nose is approached through a precartilaginous incision on the cleft side, and "V-Y" advancement of a mucocartilaginous flap is realized to restore the normal alar cartilage shape and position. The follow-up showed normal development of the ala with position maintenance without nasal stenosis in 95% of the cases. Good lip functional results were seen in 100% of the cases. Minimal skin scar revision procedures were required in 10% of the cases.
The closed method - with IMF and conservative - and the open reduction - with ORIF - brought good clinical results in an evaluation up to 6 months. We could also conclude that there is no difference in the complaint results after the open or closed treatment according to the patients' opinion.
In CT evaluation the implants of CPV have greater deformation that the S, which makes them not suitable for replacement of membranous bone in the rat skull.
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