ODR-10, an olfactory receptor protein of C. elegans, was used as a model of olfactory receptors expressed in human embryonic kidney (HEK)-293 cells as a fusing protein with 6×his-tag and rho-tag. The expression of the target protein was analyzed by RT-PCR (reverse transcription-polymerase chain reaction), Western blot and immunocytochemistry methods. The specific binding to odorant molecules of the olfactory receptor was measured using intracellular Ca 2+ assays. Then the olfactory receptor ODR-10 was immobilized onto the surface of quartz crystal microbalance (QCM) sensor to fabricate the olfactory receptor-based gas biosensor. The results demonstrated that this biosensor can detect the natural ligand of ODR-10 (diacetyl) with high sensitivity and specificity. All these results indicated that the olfactory receptor ODR-10 has been expressed successfully in the heterologous cell system and it can be used as the sensitive material in the development of biomimetic olfactory receptor-based biosensors.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.