The purpose of this study was to characterize rat adipose-derived stem cells,
induce adipose-derived stem cell tenogenesis, and analyze adipose-derived stem
cell effects on tendon repair in vivo. Adipose-derived stem cells demonstrated
an immunomodulatory, pro-angiogenic, and pro-proliferatory profile in vitro.
Tenogenesis was induced for 1, 7, 14, and 21 days with 24 combinations of growth
differentiation factor-5, 6, and 7 and platelet-derived growth factor–BB.
Adipose-derived stem cells expression of scleraxis and collagen type I increased
the most after 14 days of induction with growth differentiation factor-6 and
platelet-derived growth factor–BB. Achilles excision defects injected with
hydrogel alone (Gp2), with undifferentiated (Gp3) adipose-derived stem cells, or
tenogenically differentiated (Gp4) adipose-derived stem cells exhibited improved
tissue repair compared with untreated tendons (Gp1). Addition of adipose-derived
stem cells improved tissue cytoarchitecture and increased expression of collagen
type I and III, scleraxis, and tenomodulin. Adipose-derived stem cells
significantly improved biomechanical properties (ultimate load and elastic
toughness) over time more than hydrogel alone, while tenogenically
differentiated adipose-derived stem cells improved the mean histological score
and collagen fiber dispersion range closest to normal tendon. In addition,
tendon sections treated with GFP-adipose-derived stem cells exhibited green
fluorescence and positive GFP immunostaining on microscopy confirming the in
vivo survival of adipose-derived stem cells that were injected into tendon
defects to support the effects of adipose-derived stem cells on tissue up to
4.5 weeks post injury.
Orbitozygomatic fractures are frequently encountered in plastic surgery. Management depends on a thorough preoperative physical examination, with attention to the ophthalmologic assessment. Coronal and axial computed tomography is essential for identifying fracture extent and orbital involvement. Adequate exposure and mobilization of fracture segments is essential for successful anatomical reduction. Failure to perform effective fixation may lead to subsequent complications, such as enophthalmos and diplopia. The authors illustrate the appropriate management of orbitozygomatic fractures in an effort to reduce complications and attain aesthetically satisfying results.
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