Kiwifruit shoots can be rapidly propagated through a micro-cross section (MCS) system we established here. Optimal culture conditions were determined for different explant types, section sizes, and concentrations of inorganic salts and plant growth regulators. Rates of survival and callus formation were higher in half-strength MS salts than in full-strength MS media. Similar performance (i.e., survival and callus formation) was achieved with section sizes of either 800 pm or 1200 pm. Proliferation efficiency was greatest when explants from stem tissue were cultured on 1/2 MS supplemented with 4.5x10 3 pM 2,4-dichlorophenoxyacetic acid and 4.6x10 i pM zeatin. The number of shoots averaged 2.61 per explant, representing an efficiency of 94%. RAPD analysis revealed that the regenerated plants from our MCS system were genetically stable. These results show that the culturing of micro-cross sections from stem tissue is a powerful method for kiwifruit propagation.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.