ObjectivesTo determine the risk factors for retinal microvascular impairment on optical coherence tomography angiography (OCT-A) in type 2 diabetic patients without clinical diabetic retinopathy (DR).MethodsThis retrospective and cross-sectional study enrolled 74 diabetic patients without clinically evident DR for the study group and 34 healthy subjects for the control group. OCT-A parameters were measured to determine vascular density (VD) and the foveal avascular zone (FAZ) size in the superficial and deep capillary plexuses (SCP/DCP) of the retina. Clinical data were collected on sex, age, diabetes duration, hemoglobin A1c (HbA1c), hypertension, dyslipidemia, low-density lipoprotein cholesterol (LDL-C), estimated glomerular filtration rate (eGFR) and smoking status. Multiple linear regression analyses were performed to represent the associated clinical variables with OCT-A parameters in diabetic patients.ResultsIn comparison between the study and control groups, the VD in the SCP and DCP were significantly lower in diabetic patients compared to the controls (P = 0.022 and 0.003, respectively). The FAZ size in the SCP and DCP were significantly greater in diabetic patients compared to the controls (P = 0.035 and <0.001, respectively). In age- and sex-adjusted multiple regression analyses for the diabetic patients, dyslipidemia and hypertension were negatively associated with SCP-VD (β = -0.357, P = 0.002; β = -0.239, P = 0.039, respectively). Current smoking was correlated with lower DCP-VD (β = -0.255, P = 0.043). Greater SCP-FAZ size was associated with dyslipidemia and greater LDL-C (β = 0.254, P = 0.013; β = 0.232, P = 0.029, respectively), and greater DCP-FAZ size, with lower eGFR and greater LDL-C (β = -0.355, P = 0.004; β = 0.235, P = 0.037, respectively).ConclusionsDiabetic patients without clinical DR showed lower VD and greater FAZ size in the SCP and DCP compared to healthy controls. In diabetic patients without clinical DR, dyslipidemia and/or high LDL-C were important risk factors for retinal microvascular impairment. Hypertension, current smoking and lower eGFR also contributed to microvascular impairment.
The performance of the BacT/Alert FA Plus and FN Plus resin bottles was evaluated in comparison with that of standard aerobic (SA) and standard anaerobic (SN) bottles. Twenty milliliters of blood from adult patients was equally distributed into four types of bottles: FA Plus, FN Plus, SA, and SN. The detection of clinically significant organisms and the time to detection (TTD) were monitored for each bottle. Among the 3,103 blood culture sets that were requested, the blood volume of each bottle was over 4 ml in 1,481 sets (47.7%). Among these 1,481 sets, 158 cultures grew in the FA Plus and SA bottles, and 136 grew in the FN Plus and SN bottles. Growth in only one type of bottle was more commonly observed for the FA Plus (n ؍ 38) than for the SA (n ؍ 14) (P ؍ 0.001) bottles and for the FN Plus (n ؍ 27) than for the SN (n ؍ 10) (P ؍ 0.008) bottles. Gram-negative bacilli were more frequently isolated in the resin bottles (P < 0.05). The skin contamination rate was 1.2% in the resin bottles and the standard bottles. The mean TTD was 11.1 h in the FA Plus bottles versus 13.1 h in the SA bottles (P < 0.001) and 12.0 h in the FN Plus bottles versus 12.8 h in the SN bottles (P ؍ 0.083). Clinically significant bacteria, including Gram-negative bacilli, were isolated more frequently from the resin bottles than from the standard bottles. Clinically significant bacteria were detected faster using the aerobic resin bottles than using the standard aerobic bottles. This finding might not be applicable to the standard-practice 10-ml protocol for each bottle because the results from using a smaller volume (5 ml) might be less pronounced. Sepsis is a critical illness causing high morbidity and mortality, and blood culture is essential for diagnosing sepsis. Advancements in blood culture equipment and broth media have been made for several decades. Antibiotics are sometimes empirically administered to manage urgent septic conditions, even before collecting blood for a bacterial culture. Approximately 50% to 90% of inpatients are administered antibiotics at the time of blood collection for culture (1-3). The presence of these antibiotics in the culture bottle may inhibit the growth of microorganisms.Resin-based media are known to absorb antibiotics present in blood culture media, which is useful for the evaluation of sepsis patients who have already received antibiotics. Charcoal-based media have the same effect but might hinder the microscopic observation of Gram staining. These bottles are known to enhance the detection of microorganisms, even for sepsis patients who do not receive antibiotics, possibly by inhibiting the activities of antibodies, complement factors, or cytokines (4).We evaluated the resin-based media that were recently developed by bioMérieux Inc. (Durham, NC), namely, FA Plus and FN Plus, for patients who visited the emergency department (ED) or who were admitted to the cancer center, the surgical intensive care unit (SICU), or the medical intensive care unit (MICU).The early detection of microorg...
BackgroundBlood volume may profoundly affect the isolation of microorganisms in blood cultures. The effect of blood volume in standard anaerobic bottles of the BacT/ALERT 3D system was investigated.MethodsAdult patients who visited the emergency department and referred for blood culture (n = 824) were enrolled from June to September 2013. Two sets of blood cultures were obtained from each patient. One set consisted of 5 mL that was collected in a standard aerobic bottle (SA5), 5 mL that was collected in a standard anaerobic bottle (SN5), and 10 mL that was collected in a standard anaerobic bottle (SN10). The growth of clinically significant pathogens and the time to detection (TTD) were compared between the SN5 and SN10 samples.ResultsIncreasing the volume of blood collected from 5 to 10 mL yielded a 14.7% improvement in the isolation of microorganisms. There was a statistically significant difference in the isolation of pathogens (14 vs. 30, P = 0.023) between the SN5 and SN10 samples. Gram-positive microorganisms were detected earlier in the SN10 samples than the SN5 samples (P = 0.052). The mean TTD of all pathogens was 13.5 h for the SN5 samples and 12.9 h for the SN10 samples (P = 0.099).ConclusionIncreased blood volume in the SN bottle yielded a significantly higher pathogen detection rate. However, there was no difference in the frequency of earlier detection or TTD between the SN5 and SN10 samples.
Vitamin D concentration is known to correlate with various parameters. A major source of vitamin D is synthesis in the skin; thus, the duration of sun exposure is known to correlate with serum vitamin D levels. We compared serum levels of vitamin D between 2 occupational groups in Korea: the fisherman group, the most sun-exposed, and the general occupation group, relatively less sun-exposed. This study was conducted on 140 healthy fishermen and 140 healthy residents with various occupations, all of whom resided in the southernmost region of Korea, from June to August 2016. We compared serum concentrations of 25-hydroxyvitamin D (25(OH)D) in both occupation groups by gender and age and suggested vitamin D reference interval for the region. Mean serum 25(OH)D concentrations in the general occupation and fisherman group were 13.60 ± 6.43 and 23.74 ± 8.88 ng/mL, respectively. Mean serum concentration of 25(OH)D was 1.7 times higher in the fisherman group compared with the general occupation group, which was statistically significant (P < .001). Approximately 98% of subjects in the general occupation group and 78% of subjects in the fisherman group demonstrated either vitamin D insufficiency or deficiency. Calculated serum 25(OH)D reference interval for all subjects in our study was 3.8 to 44.4 ng/mL. Despite exposure to a large amount of sunlight, 78% of subjects in the fisherman group presented with either vitamin D deficiency or insufficiency. By taking laboratory measurements of serum 25(OH)D concentrations in fisherman, who were expected to have the highest vitamin D concentrations in Korea, this study could be epidemiologically useful.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.