The human tyrosinase ectodomain has been expressed in Escherichia coli as a soluble form and purified by immobilized metal affinity column chromatography. The ectodomain exhibited tyrosinase activities toward the hydroxylation and oxidation reactions. Biochemical properties of the ectodomain appeared to be distinct from those of the human tyrosinase, although common features were retained.
Surface modification of silica spheres with 3-(Trimethoxysilyl)propylmethacrylate (TMSPM) has been performed at ambient condition. However, the FTIR spectra and field emission scanning electron microscope (FESEM) images show no evidence of the surface modification. The reaction temperatures were varied from 60 to 80 °C with various reaction periods. Small absorption shoulder of the C=O stretching vibration was at 1700 cm -1 , and slightly increased with the increase of the reaction time at 60 °C. The clear absorption peak appeared at 1698 cm -1 for the spheres reacted for 80 min at 70 °C and shifted toward 1720 cm -1 with the increase the reaction time. Strong absorption peak showed at 1698 cm -1 and shifted toward 1725 cm -1 with the increase of the reaction time at 80 °C. The spheres were dispersed to methanol and added photoinitiator (Irgacure-184). The solution was poured to a patterned glass substrate and exposed to the 254 nm UV-light during a self-assembly process. A large area and crack-free silica sphere film was formed. To increase the mechanical stability, a cellulose acetate solution was spin-coated to the film. The film was lift-off from the glass substrate to analyze the surface nanostructures. The surface nanostructures were maintained, and the film is stable enough to use as a mold to duplicate the nanopattern and flexible.
The adaptive wavelet method is studied for the enhancement of computational efficiency of three-dimensional flows. For implementation of the method for three-dimensional Euler equation, wavelet decomposition process is introduced based on the previous two-dimensional adaptive wavelet method. The order of numerical accuracy of an original solver is preserved by applying modified thresholding value. In order to assess the efficiency of the proposed algorithm, the method is applied to the computation of flow field around ONERA-M6 wing in transonic regime with 4th and 6th order interpolating polynomial respectively. Through the application, it is confirmed that the three-dimensional adaptive wavelet method can reduce the computational time while conserving the numerical accuracy of an original solver.
Photoluminescence of disperse red19 (DR19) have been investigated with various poling field strengths. Two step synthetic processes were employed to employ the DR19 to the TiO 2 solgel including a urethane bond formation between a DR19 (OH) and a 3isocyanatopropyl triethoxysilane (ICPTES, N=C=O) and the hydrolysis of the ethoxy group from the ICPTES and condensation reaction between silanol groups from ICPTES and TiO 2 . The composite was coated to the ITO coated glass substrate. Corona poling were performed before drying the composite with various electric field strengths. The photoluminescence also decreased as the poling field increased. There is long luminescence tail for the poled TiO 2 DR19 film compared with unpoled TiO 2 DR19 film. The luminescence long tail indicates that the selftrapped excitons and small polarons were generated when the film poled relatively low electric field.
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