A bstractMethyl-β-cyclodextrin, a cyclic oligosaccharide know n for its interaction w ith the plasm a m em brane induces several events in cells including cell growth and anti-tum or activity. In this study, w e have investigated the possible role of cyclooxygenase 2 (COX-2) in cell growth arrest induced by m ethyl-β-cyclodextrin in Raw264.7 macrophage cells. M ethyl-β-cyclodextrin inhibited cell grow th and arrested the cell cycle, and this cell cycle arrest reduced the population of cells in the S phase, and concom itantly reduced cyclin A and D expressions. M ethyl-β-cyclodextrin in a dose-and tim e-dependent manner, also induced COX-2 expression, prostaglandin E 2 (PG E 2 ) synthesis, and CO X-2 prom oter activity. Pretreatm ent of cells w ith NS398, a CO X-2 specific inhibitor com pletely blocked PG E 2 synthesis induced by m ethyl-β-cyclodextrin, how ever inhibition on cell proliferation and cell cycle arrest w as not effected, suggesting nonas so cia tio n o f C O X -2 in th e ce ll cy cle arres t. These results suggest that m ethyl-β-cyclodextrin induced cell growth inhibition and cell cycle arrest in Raw 264.7 cells m ay be m ediated by cyclin A and D1 expression.
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