The present study using array-CGH analyses sought a deeper elucidation of the specific genomic alterations related to ULMS. The high resolution of array-CGH combined with human genome database would give a chance at identifying relevant target genes.

Abstract. Ovarian cancer is the most common cause of diseaserelated death in women globally. Detection of ovarian cancer using new biomarkers is necessary for early diagnosis. To date, there have been no obvious biomarkers for ovarian cancer detection in the incipient stage. In this study, we discovered potential diagnostic serological biomarkers for ovarian cancer using the Experion™ automated electrophoresis system. Sera from 14 healthy women and 84 ovarian cancer patients at stages I-IV were applied to the Experion to compare the protein expression levels. To examine the protein expression pattern of Experion data, proteins in the samples were resolved using 10 and 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and visualized by silver staining. The candidate biomarkers elevated in ovarian cancer were purified and determined using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. α-2-macroglobulin (173.7 kDa), ceruloplasmin (147 kDa), inter-α-trypsin inhibitor family heavy chain-related protein (126 kDa), C-1 inhibitor (115.2 kDa) and hemoglobin α/β (14.4 kDa were overexpressed in the ovarian cancer sera. This study documents a novel way to measure ovarian cancer or cancer-related proteins for biomarkers using the Experion assay system, which should be easily adaptable for high-throughput diagnosis to establish databases of ovarian cancer for clinical applications.

Abstract. Cervical cancer is a serious disease that threatens the health of women worldwide. This study compared the sensitivities and false-positive rates of cervical cytology (Pap smear), human papilloma virus (HPV) DNA test, cervicography, first double-combined testing (cervical cytology and HPV DNA test), second double-combined testing (cervical cytology and cervicography) and triple-combined testing (cervical cytology, HPV DNA test and cervicography). The study included 261 patients screened for uterine cervical cancer. All women simultaneously underwent cervical cytology, HPV DNA test and cervicography for uterine cervical cancer screening and colposcopically directed biopsy for diagnostic evaluation. The triple-combined testing was consistently the most sensitive among the cervical screening tests. The second double-combined testing, with a sensitivity rate of 98.1% was more sensitive than the first double-combined test (92.3%). However, cervical cytology was most specific (93.5%) and showed the highest positive predictive value (77.8%). The sensitivity of cervical cytology was markedly improved in combination with HPV DNA test and cervicography. Thus, the triple-combined testing, which improves the high false negativity of cervical cytology, may be an effective tool in uterine cervical cancer screening, pending confirmation of the effectiveness in a mass screening study.

The detection of human papillomavirus (HPV) of female genital tract is very important, several HPV DNA detection methods have been developed during the last decade. In previous report, we introduced luminex-based HPV genotyping assay that is combined PCR amplification. In this study, we compared performances to detect high risk HPV types in hybrid capture 2 (HC II), DNA Chip, and luminex-based assay. A total of 330 cervical swabs from Cervical Cancer Early Diagnostic Clinic, Seoul St. Mary's Hospital were investigated. DNA was extracted from a single sample and HPV DNA detection assays were performed on the same specimen with 3 strategies and confirmed by DNA sequencing. Among 330 cervical swabs, 230 were HPV positive, 100 were negative. HPV detection rates of Luminex, HC II and DNA Chip in 330 specimens were 65.2%, 61.2% and 68.8%, respectively. The results of DNA sequence analysis, accuracy of Luminex, HC II and DNA Chip was 91.2%, 82.7% and 80.9%, respectively. HPV DNA infection slightly increase with severity of disease in cytologic and histologic diagnosis, there is no correlation between HPV, because HPV DNAs were detected in many samples with normal in this study. Conclusively in HPV detection comparison, DNA Chip had relatively high detection rate than Luminex and HC II, but Luminex had the highest accuracy for HPV DNA detection. These results indicate that the Luminex-based assay has more accurate performance than DNA Chip and HC II assay in the detection of HPV infections. We suggest that Luminex-based assay may be useful method for detecting of HPV infection clinically. Note: This abstract was not presented at the AACR 101st Annual Meeting 2010 because the presenter was unable to attend. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 824.