Lycopersicon pennellii Corr. (D'Arcy) an insect-resistant, wild tomato possesses high densities of glandular trichomes which exude a mixture of 2,3,4-tri-O-acylated glucose esters that function as a physical impediment and feeding deterrent to small arthropod pests. The acyl moieties are branched C4 and C5 acids, and branched and straight chain C10, C11, and C12 acids. The structure of the branched acyl constituents suggests that the branched chain amino acid biosynthetic pathway participates in their biosynthesis.[14C]Valine and deuterated branched chain amino acids (and their oxo-acid derivatives) were incorporated into branched C4 and C5 acid groups of glucose esters by a process of transamination, oxidative decarboxylation and subsequent acylation. C4 and C5 branched acids were elongated by two carbon units to produce the branched CIO-C12 groups. Norvaline, norleucine, allylglycine, and methionine also were processed into acyl moieties and secreted from the trichomes as glucose esters. Changes in the acyl composition of the glucose esters following sulfonylurea herbicide administration support the participation of acetohydroxyacid synthetase and the other enzymes of branched amino acid biosynthesis in the production of glucose esters.

their plant products at various points in seed and food production (Beever and Kemp, 2000). Grain handlers In light of (i) public concern with the safety of genetically modified must develop the capability to evaluate the presence or crops, (ii) the issue of food labeling, and (iii) governmental regulation of transgenic plants, quality control (QC) monitoring of the process proportion of transgenic grain in truckloads and other of Transgenic Seed Product Development (TSPD) is essential to grain lots as nontransgenic grain takes on identity-preprotecting research investments, maintaining compliance with governserved status. Likewise, food processors need to effecmental regulations, and ensuring customer satisfaction. The primary tively monitor supply chains within identity preservation goal of QC monitoring in TSPD is to ensure the authenticity (transsystems to maintain compliance with the labeling regenic event identity and purity) of seed materials used in product quirements demanded by some markets. Furthermore, testing, in the development of regulatory data packages for governthe efficient evaluation of the type of any transgenic mental review, and to develop seed volumes for commercial release. grain present in supply chains is important to ensure Monitoring is performed to confirm the presence of the presumed compliance with governmental regulation. The auditing transgenic event(s) and the absence of all others. Sophisticated QC requirement actually begins with the seed companies, strategies formulated to monitor the product development process and to maintain quality standards in the manufacturing industry can which develop crop seed products for retail markets. serve as a foundation in devising efficient strategies tailored to meet Presently, seed certification requirements are expanding needs in the seed industry. A number of considerations in the design The Process of Transgenic SeedPublic concern with the safety of transgenic crops, Product Developmentthe issue of food labeling, as well as governmental regulation of transgenic plants by U.S. and internationalThe general process of TSPD encompasses a number agencies has spawned much debate over measures reof steps from transformation to new product launch, quired to assess genetic components of these plants and Abbreviations: BC1, generation resulting from the first backcross to the recurrent parent (BC2-BC6 follow the same convention with the specific generation designated)

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