Studies have reported that dental resin-based materials release substances which have biological liabilities. However, some current methods for detecting these substances may not be adequate to detect biologically relevant concentrations. In the current study, we hypothesized that resin-based materials exhibit cytotoxic effects and alter cellular function in vitro when high-pressure liquid chromatography (HPLC-UV detection) cannot detect any release of substances. We further hypothesized that this release continues even after aging the samples in artificial saliva. Five types of composite or compomer materials (Z-100, Tetric Ceram, Dyract AP, Solitaire, and Clearfil AP-X) and one organically modified ceramic material (Definite) were tested after aging in artificial saliva for 0, 7, or 14 days. Cytotoxicity was assessed using direct contact with fibroblasts and measurement of succinic dehydrogenase activity after 48 h of exposure post aging. Release of substances from the materials was assessed using HPLC with UV detection. Altered cellular function was estimated by measuring proliferation of MCF-7 cells with sulforhodamine staining. HPLC showed that whereas initial release of substances was higher without aging, this release dropped significantly after 7 or 14 days of aging, and was equivalent to the Teflon controls after 14 days for four of the materials (Tetric Ceram, Definite, Solitaire, and Clearfil AP-X). Without aging in saliva, all materials had cytotoxicities > 50% of the Teflon negative controls. After 14 days of aging, all materials except the Definite continued to show severe cytotoxicity. Only the Definite could be tested for its ability to alter cellular function because of the continuing toxicity of the other materials. This modified ceramic material caused a significant proliferative effect on the MCF-7 cells indicating that sufficient substances were released to alter cellular function. We concluded that all of these commercially available resin-based dental materials continue to release sufficient components to cause lethal effects or alter cellular function in vitro even after 2 weeks of aging in artificial saliva.

Purpose: This research investigated composite depths of cure using a variety of light-curing units and exposure protocols. Materials and Methods:Composite (Herculite XRV, shade A2, Kerr, Orange, California) was exposed in opaque compules to conventional quartz tungsten halogen (QTH) units, soft-start units, high-intensity QTH and plasma arc (PAC) curing lights, and one argon laser. Cured compules were sonicated to remove uncured composite and were sectioned and polished along the long axis to expose cured composite. Knoop hardness was measured 0.5 mm from the irradiated, top surface and then at 1.0 mm and in 1.0-mm increments until reliable readings could no longer be obtained. Hardness values were compared by analysis of variance at similar depths within a specific curing-light classification, using the hardness of the standard 40-second conventional QTH exposure as comparison (Dunnett's t-test). Depth of cure was defined as the deepest hardness value found equivalent to that at 0.5-mm depth for a specific curing light and scenario. Results: Conventional QTH lights provided similar hardness profiles. At 2-mm depth, use of a different unit or curing tip made no difference in hardness compared with the standard. At this depth, soft-start (pulse-delay and stepture) methods yielded hardness similar to that of the standard. High-intensity QTH lights provided similar hardness at 2-mm depth in 10 seconds to that of the standard 40-second exposure. Plasma arc exposure for less than 10 seconds produced inferior hardness compared with the standard. A 10-second PAC and a 5-second laser exposure gave hardness at 2-mm depth equivalent to that of the 40-second standard. Depth of cure for almost all curing scenarios was not greater than 2 mm. CLINICAL SIGNIFICANCESimilar-type conventional QTH lights with different tip diameter (8 and 12 mm) provide similar composite cure characteristics. Soft-start techniques provide similar cure profiles to those achieved with conventional QTH technique when used according to manufacturer's recommendations. High-intensity QTH units and the argon laser can reduce exposure time while providing composite with similar hardness to that of conventional QTH curing. Plasma arc exposure should be at least of 10 seconds duration to provide hardness equivalent to that achieved with conventional 40-second QTH exposure. Even with consideration of high-intensity curing units, composite increments should still be no greater than 2 mm to provide homogeneous hardness. 23. K e k y WP, Blankanau RJ, P o d GL, Barkmeier WW, Stormberg EF. Power and time requirements for use of the argon laser to polymerize composite resins. J Clin Laser Med Surg 1992; 10:273-278. 24. Ferracane JL, Mitchem JC, Condon JR, Todd R. Wear and marginal breakdown of composites with various degrees of cure. J

New polymeric calcium phosphate cement composites (CPCs) were developed. Cement powder consisting of 60 wt% tetracalcium phosphate, 30 wt% dicalcium phosphate dihydrate, and 10 wt% tricalcium phosphate was combined with either 35% w/w poly methyl vinyl ether maleic acid or polyacrylic acid to obtain CPC-1 and CPC-2. The setting time and compressive and diametral tensile strength of the CPCs were evaluated and compared with that of a commercial hydroxyapatite cement. In vitro cytotoxicity and in vivo biocompatibility of the two CPCs and hydroxyapatite cement were assessed. The setting time of the cements was 5–15 min. CPC-1 and CPC-2 showed significantly higher compressive and diametral strength values compared to hydroxyapatite cement. CPC-1 and CPC-2 were equivalent to Teflon controls after 1 week. CPC-1, CPC-2, and hydroxyapatite cement elicited a moderate to intense inflammatory reaction at 7 days which decreased over time. CPC-1 and CPC-2 show promise for orthopedic applications.

Compomers and resin‐modified glass ionomers have been developed to improve the physical properties of traditional glass ionomer cements. This project compared the toothbrush wear‐resistance of three compomers (Compoglass, Dyract, Hytac) and three resin‐modified glass ionomer restorative materials (Fuji II LC, Photac‐Fil, Vitremer) to that of two resin‐based composites (Herculite XRV, Silux Plus). Specimens (n = 7) were prepared according to manufacturers' instructions and stored in a humidor for 48 hours prior to testing. The specimens were subjected to 120,000 strokes at 1.5 Hz, using a brush‐head force of 200 g on a Manly V‐8 cross‐brushing machine. The slurry contained a 50:50 (w/w) mixture of toothpaste and deionized water. Abrasion‐resistance was calculated by measuring specimen mass‐loss prior to and subsequent to brushing. The data were analyzed using a one‐way analysis of variance (ANOVA) and the Tukey‐Kramer post‐hoc test. Significant differences (p±.0001) in mass‐loss were found, and loss ranged from 0.013 ± 0.003 g (Hytac) to 0.061 ± 0.009 g (Compoglass). No correlation (p= .959) between wear‐resistance and experimentally determined filler content existed. This study showed that all but one hybrid resin‐ionomer type material exhibited a resistance to toothbrush wear that was as good as or better than that of the two traditional resin‐based composite materials.

Objectives: Balloon dilation is generally considered first-line treatment for airway stenosis. Some dilation systems utilize a compliant balloon that can conform around rigid structures. Others use a noncompliant balloon that does not conform, allowing for dilation of more rigid stenoses. We hypothesized that subglottic dilation with a noncompliant balloon increases the likelihood of fracture of the cricoid when compared to a compliant balloon. Methods: Three fresh human cricoid cartilages were placed in a universal testing system to determine the expansile force necessary for cricoid fracture. Using these data, a 3D printer was used to construct a synthetic cricoid model possessing near identical physical characteristics to the human cricoid. Simulated dilation was then performed on the model using a compliant and a noncompliant balloon. Results: Human cricoid fracture occurred at 97.25 N (SD = 8.34), and the synthetic cricoid model fractured at 100.10 N (SD = 7.32). Both balloons fractured the model in every replicate experiment. Mean balloon internal pressure at fracture was 7.67 ATM (SD = 1.21) for the compliant balloon and 11.34 ATM (SD = 1.29) for the noncompliant balloon. Conclusions: These data show that fracture of the cricoid is a valid concern in balloon dilation procedures where the balloon spans the subglottis. Furthermore, the hypothesis was rejected in that the compliant balloon system was at least as likely to fracture the cricoid model as the noncompliant.

The release of elements from dental alloys has been linked to alloy biocompatibility. Much of the research measuring elemental release has been done in vitro under passive conditions. The current study supplements a previous report that measured elemental release from dental alloys during and after the equivalent of 1 week of toothbrushing. In the current study, toothbrushing times were extended to the equivalent of 2 years, and elemental release was measured during and after brushing, with and without toothpaste. The results showed that for the major classes of dental alloys, brushing alone caused no significant elemental release during the brushing, and only minor increases after brushing. Brushing with toothpaste caused significant increases in elemental release for all elements of all alloys, but the largest increases were for the two nickel-based alloys. Nickel released during brushing with toothpaste reached 600-800 microg/cm(2) of alloy surface. Both beryllium-containing and non-beryllium-containing nickel-based alloys behaved similarly, refuting claims that non-beryllium alloys are superior in this regard. Thus, brushing with toothpaste under these extended in vitro conditions appears to increase the biological liabilities from elemental release for all alloys, but primarily for nickel-based alloys.

Silorane-and high filled-based "lowshrinkage" resin composites: shrinkage, flexural strength and modulus Abstract: This study compared the volumetric shrinkage (VS), flexural strength (FS) and flexural modulus (FM) properties of the low-shrinkage resin composite Aelite LS (Bisco) to those of Filtek LS (3M ESPE) and two regular dimethacrylate-based resin composites, the microfilled Heliomolar (Ivoclar Vivadent) and the microhybrid Aelite Universal (Bisco). The composites (n = 5) were placed on the Teflon pedestal of a videoimaging device, and VS was recorded every minute for 5 min after 40 s of light exposure. For the FS and FM tests, resin discs (0.6 mm in thickness and 6.0 mm in diameter) were obtained (n = 12) and submitted to a piston-ring biaxial test in a universal testing machine. VS, FS, and FM data were submitted to two-way repeated measures and one-way ANOVA, respectively, followed by Tukey's post-hoc test (α = 5%). Filtek LS showed lower VS than did Aelite LS, which in turn showed lower shrinkage than did the other composites. Aelite Universal and Filtek LS exhibited higher FS than did Heliomolar and Aelite LS, both of which exhibited the highest FM. No significant difference in FM was noted between Filtek LS and Aelite Universal, while Heliomolar exhibited the lowest values. Aelite LS was not as effective as Filtek LS regarding shrinkage, although both lowshrinkage composites showed lower VS than did the other composites. Only Filtek LS exhibited FS and FM comparable to those of the regular microhybrid dimethacrylate-based resin composite.

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