In this study, the intravitreal use of bevacizumab resulted in improvement of mf-ERG macular function responses and relatively stable G-ERG responses. The macular electrophysiologic response suggests that macular function improves with treatment. G-ERG suggests that there is no significant measurable photoreceptor toxicity with the use of intravitreal bevacizumab over the short term.
Plasma transthyretin (TTR) is synthesized in the liver and is the source for visceral amyloid deposits in TTR amyloidosis. However, TTR is also synthesized in the retinal pigment epithelium of the eye and choroid plexus of the brain. It has been postulated that vitreous amyloid, which is associated with approximately 20% of the known amyloidogenic TTR mutations, results from local synthesis of TTR in the eye. In order to elucidate if differences in amyloid between organs exists, we have analyzed vitreous and cardiac amyloid fibrils in Ile84Ser TTR patients for comparison. Analysis of guanidine hydrochloride solubilized protein from isolated vitreous and cardiac amyloid fibrils indicated that the amyloid TTR in both organs is highly proteolyzed with minor amounts of intact TTR present. While vitreous protein was amenable to direct Edman sequence analysis, cardiac protein gave low yields indicating it was mostly N-terminally blocked or inaccessible to Edman degradation. While vitreous contained major 11 kDa and minor 9 kDa fragments, cardiac contained at least three major fragments of 7-11 kDa. Vitreous protein was cleaved between Lys48-Thr49, while cardiac protein was cleaved at multiple sites in the residue 46-52 region. While deposits in both tissues were enriched in variant TTR, vitreous fibrils contained more variant protein than cardiac fibrils (80-89% vs. 60-65% Ser84TTR). These differences suggest that the mechanism or pathway of fibril formation may differ in various tissues.
The "dichromate" paper developed by Ripley, Clingenpeel, and Hum was used successfully in our laboratory for conversion of nitric oxide to nitrogen dioxide at concentrations up to the 1 ppm level. When this paper was used for synthetic mixtures with nitric oxide concentrations in the range of 1-3 ppm, the analyses were erratic and values were lower than calculated. After a number of modifications of the procedures for preparing and using the impregnated paper, a modification of the "dichromate" paper was achieved that reduced scatter in the NO analysis and improved conversion efficiency. The efficiency and reproducibility of the modified paper are good enough that efficiency factors may be established to correct data obtained over a period of at least 6 hours of continuous use at relative humidities (RH) near 50%.
This paper relates how thermochemical principles can be applied in predicting the heat of combustion of prepared solid waste samples, knowing their ultimate analysis. The resulting formula produces data whose variation from average experimental value is similar in magnitude to the variation between duplicate experiments.
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