DNA secondary structure plays an important role in biology, genotyping diagnostics, a variety of molecular biology techniques, in vitro-selected DNA catalysts, nanotechnology, and DNA-based computing. Accurate prediction of DNA secondary structure and hybridization using dynamic programming algorithms requires a database of thermodynamic parameters for several motifs including Watson-Crick base pairs, internal mismatches, terminal mismatches, terminal dangling ends, hairpins, bulges, internal loops, and multibranched loops. To make the database useful for predictions under a variety of salt conditions, empirical equations for monovalent and magnesium dependence of thermodynamics have been developed. Bimolecular hybridization is often inhibited by competing unimolecular folding of a target or probe DNA. Powerful numerical methods have been developed to solve multistate-coupled equilibria in bimolecular and higher-order complexes. This review presents the current parameter set available for making accurate DNA structure predictions and also points to future directions for improvement.

Empirical studies of determinants of new firm formation to date have tended to yield diverse and even contradictory results. Three primary reasons for this have been advanced: (1) the paucity of suitable micro-level data (2) the failure to control for amorphous time- and place-specific influences that defy specification, and (3) the use of estimation techniques that do not handle adequately the effects of heteroscedasticity. This paper addresses all of these shortcomings by employing a unique data set composed of annual data on localized firm entry, exit and a variety of predictor variables that has been analyzed to yield heteroscedasticity-corrected estimates while controlling for unspecified place- and period-specific influences. We test a variety of models seeking to explain patterns of new firm formation in terms of macroeconomic, demographic, and labor market processes, patterns of industrial restructuring, availability of local financial capital, and local public sector spending. Our results suggest that regional patterns of new firm formation can be explained by variation in unemployment change rates, mean establishment size, prior firm entry and exit dynamics, and the availability of local financial capital. We find no evidence of influence attributable to population or income dynamics, unemployment level, or local government spending. Copyright Springer-Verlag 2004

The thermodynamic contributions of rA·dA, rC·dC, rG·dG and rU·dT single internal mismatches were measured for 54 RNA/DNA duplexes in a 1 M NaCl buffer using UV absorbance thermal denaturation. Thermodynamic parameters were obtained by fitting absorbance versus temperature profiles using the curve-fitting program Meltwin. The weighted average thermodynamic data were fit using singular value decomposition to determine the eight non-unique nearest-neighbor parameters for each internal mismatch. The new parameters predict the ΔG°37, ΔH° and melting temperature (Tm) of duplexes containing these single mismatches within an average of 0.33 kcal/mol, 4.5 kcal/mol and 1.4°C, respectively. The general trend in decreasing stability for the single internal mismatches is rG·dG > rU·dT > rA·dA > rC·dC. The stability trend for the base pairs 5′ of the single internal mismatch is rG·dC > rC·dG > rA·dT > rU·dA. The stability trend for the base pairs 3′ of the single internal mismatch is rC·dG > rG·dC >> rA·dT > rU·dA. These nearest-neighbor values are now a part of a complete set of single internal mismatch thermodynamic parameters for RNA/DNA duplexes that are incorporated into the nucleic acid assay development software programs Visual oligonucleotide modeling platform (OMP) and ThermoBLAST.

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Prisoners' attitudes toward components of the legal and judicial systems are reviewed and analyzed with data collected from prisoners in their first several days of incarceration. These data are analyzed within subgroups of the prison population. In general, attitudes toward the police are negative, attitudes toward the law and the judicial system are mixed, and attitudes toward lawyers are positive. However, preprison and prison status factors influence the attitudes held by prisoners, and these different patterns of influence are discussed.