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Abstract-

Carica papaya leaves have long been used in some folk remedies to treat cancer. This folk remedy originated from Queensland - Australia from 1978 (Harald W. Tietze, 1997). In Vietnam, there are some successful cases of using Carica papaya leaves for cancer treatment even though this mediation do not always work out. In this study, the soluble substances in water (aqueous extract), the carotenoid and the polar substances (polar extract) were extracted from Carica papaya leaves grown in Vietnam; the bioassays such as cytotoxicity on some tumor cell lines as well as antioxidant activity were also tested with obtained extracts. The results showed that all obtained extracts had antioxidant activity stronger than the control substance - vitamin C at the same tested concentration. At concentration of 100 μg/ml, the inhibitive activity on lung cancer cell line LU-1 of obtained extracts was highest, from 43.47% (aqueous extract) to 62.88% (polar extract). On carcinoma cell line KB, inhibitive ability of extracts was lower: 2.8% (polar extract) and 20.6% (aqueous extract). On the breast cancer cells MCF7, inhibitive ability of above extracts was quite high, from 33.95% to 56.19%. On the leukemia cell line HL 60, inhibitive ability of these extracts was also high, from 39.56 to 60.64%. In addition, the result pointed out that on stem cells isolated from mouse embryos (ESC), the obtained extracts was not toxic to healthy cells of this line. This suggested that extracts from Carica papaya leaves has the potential to become one of the natural substance products that can support cancer treatment in certain cases.

Isoflavones in soybean including genistein, daidzein, glycitein and their acetyl and malonyl derivatives that have week estrogenic activity, so they are called phytoestrogen. There are many of papers publishing the research results about isoflavones from soybean seed and soybean sprout. They can balance estrogenic hormone and support woman to prevent menopause symptoms. Therefore, they have other precious biological activities such as anti - cancer, anti – oxidant and anti - osteomalacia. On the market, we can find many of functional foods of isoflavones from soybean seed and soybean sprout. Soybean germ is the part of soybean seed contains highest isoflavones, and the isoflavones content in soybean germ higher than that in the whole soybean seed about 5 times. In this paper, the total isoflavones were extracted from soybean germ. Five of influent factors on the isoflavone extraction are solvent concentration, extraction temperature, extraction time, extraction pH and the material/solvent ratio were optimized. The content of isoflavones in soybean germ material is from 1.8 to 2 percent. After extraction with optimized conditions, we obtain the total crude isoflavones extract that contains 3.8% of isoflavones.