Bisphenol A (BPA) is an endocrine-disrupting compound found in the environment as well as in the plastic food containers for human use. Most impact of this compound to animals is acute toxicity to the liver of young individuals, but little is known about chronic toxicity to the adult liver. Here we report that, when adult zebrafish were exposed to various concentration of bisphenol A for 60 days, we have the number of liver protein spots in 2D-PAGE gels was reduced to 15-25%. Protein identification by HPLC-ESI (electron spray ionization) revealed that sixteen 2D-PAGE spots were remarkably altered and majority of them are related to metabolic energy system; e.g., liver basic L-lactate dehydrogenase B-B chain, ATP synthase subunit beta, and intracellular fatty acid-binding proteins. Besides, heat shock protein 60kDa 1 (HSPD1), which is known to associate with inflammation, was over-expressed in fish liver after exposure to 100 µg/l BPA, but not at lower concentrations of BPA. Thus, chronic exposure to environmental BPA may cause liver dysfunctions. Sixteen hepatic proteins identified in this study would be potential biomarkers for chronic BPA toxicity. Citation: Ngo Thi Mai, Do Hong Lan Chi, Le Phi Nga, 2017. A proteomic analyses to assess the effects of chronic exposure of bisphenol a to adult zebrafish (Danio rerio). Tap chi Sinh hoc, 39(3): 333-341. DOI: 10.15625/0866-7160/v39n3.98995. *Corresponding author: lephinga1@hotmail.com, lephinga@hcmut.edu.vn Received 12 December 2016, accepted 20 August 2017
An experimental study to use a pilot vegetated submerged bed (VSB) wetland for the advanced treatment of effluent from the central wastewater treatment plant (CWWTP) of an industrial zone was carried out. The pilot VSB wetland included reeds (Phragmites australis), cattail (Typha orientalis), and blank cells in parallel. The constructed wetland was observed to be a suitable measure for wastewater reuse via the high performance of organic matter, turbidity removal, and detoxification. At loading rates of up to 250 kg chemical oxygen demand (COD) ha-1d-1, both cells with emergent plants obtained high efficiency of contaminant removal. Suspended solids (SS) and turbidity removal reached 67-86% and 69-82%, respectively. The COD removal efficiencies of the reed and cattail cells at a loading rate of 130 kg COD ha-1d-1were 47 and 55%, respectively. At a high loading of 400 kg COD ha-1d-1, the toxicity unit (TU) reduced from 32-42 to 4.9 and 4.2 in the effluent of the cattail and reed cells, respectively. Especially at loadings of 70, 130, and 185 kg COD ha-1d-1, the effluent TU was less than 3.0, corresponding to a non-toxic level to the ecosystem. The effluent quality met industrial or landscaped wastewater reuse at these loading rates.
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