Uridine insertion/deletion (U-indel) editing of mitochondrial mRNA, unique to the protistan class Kinetoplastea, generates canonical as well as potentially non-productive editing events. While the molecular machinery and the role of the guide (g) RNAs that provide required information for U-indel editing are well understood, little is known about the forces underlying its apparently error-prone nature. Analysis of a gRNA:mRNA pair allows the dissection of editing events in a given position of a given mitochondrial transcript. A complete gRNA dataset, paired with a fully characterized mRNA population that includes non-canonically edited transcripts, would allow such an analysis to be performed globally across the mitochondrial transcriptome. To achieve this, we have assembled 67 minicircles of the insect parasite Leptomonas pyrrhocoris, with each minicircle typically encoding one gRNA located in one of two similar-sized units of different origin. From this relatively narrow set of annotated gRNAs, we have dissected all identified mitochondrial editing events in L. pyrrhocoris, the strains of which dramatically differ in the abundance of individual minicircle classes. Our results support a model in which a multitude of editing events are driven by a limited set of gRNAs, with individual gRNAs possessing an inherent ability to guide canonical and non-canonical editing.

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Using the implemented objects as an example, the advantages of using laser scanning at inspecting technically complex structures and strain monitoring are shown. The practice of using scanning while protecting a cultural heritage object, preparing the initial data for BIM modeling, for examining artificial structures and determining deformations of a road embankment is described. Examples of sharing software products for processing and interpreting laser scanning results are presented. The description of creating a survey justification for working on ground-based laser scanning is given. The advantages of introducing laser scanning technology into theenterprise’s activities are shown. Real examples show that the use of ground-based laser scanning enables restoring lost project documentation, determining the deformation of structural elements, monitoringthe current shape of the embankments, evaluating the volume of sedimentary rocks and solving other problems. It is noted that the use of laser scanning can significantly reduce the time required for field work, which is especially important when working at objects with a limited access mode.

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