This study describes the chemical composition and the antibacterial, antifungal and antioxidant activities of the essential oil extracted from aerial parts of the Algerian Eryngium tricuspidatum L., obtained by hydrodistillation and analysed by using the combination of gas chromatography (GC) and GC/mass spectrometry. A total of 63 compounds were identified accounting for 93.1% of the total oil. Chemical composition of oil was characterised by a high proportion of oxygenated sesquiterpenes (49.6%) among which α-bisabolol (32.6%) was the predominant compound. The sesquiterpene hydrocarbons represent the second major fraction (31.9%) with α-curcumene (6.5%) being the predominant one. Antibacterial and antifungal activities of the oil were tested using the micro-well determination of minimum inhibitory concentration (MIC) assay against eleven bacteria and two Candida species. It was found that the aerial parts of E. tricuspidatum exhibited interesting antibacterial and anticandidal activities (MIC = 9 μg/mL against several strains of bacteria and MIC = 4.6 μg/mL against Candida albicans). The antioxidant effect of this oil was evaluated using the 2,2-diphenyl-l-1-picrylhydrazil (DPPH) and ferric reducing antioxidant power (FRAP) assays. Results revealed significant activities (DPPH method: IC₅₀ = 510 μg/mL; FRAP assay: reducing power of oil increases from 0.0188 at 5 μg/mL to 0.5016 at 1000 μg/mL).
The aim of the present study is to investigate the chemical composition, antibacterial and antioxidant activities of three essential oils extracted by hydrodistillation from the aerial parts of Artemisia arborescens L., which was collected from three different regions near Tlemcen city in the West Northern of Algeria: Beni Snous, Bidar and Chetouane. The chemical composition was investigated using both capillary gas chromatography (GC) and gas chromatography-mass spectrometry (GC/MS) techniques. Fifty-two compounds were detected, a total of fifty compounds, representing 81.8 to 90.2% of the total oils were identified in the three samples of essential oils. The essential oils of A. arborescens were rich in camphor (Beni Snous: 72.2%, Bidar: 50.3% and Chetouane: 32.8%). The present composition of the essential oils, with camphor as the only most abundant component, was considered as a new chemotype of A. arborescens growing in West Northern of Algeria. Antibacterial activity of the essential oils against gram-positive and gram-negative bacteria, was tested using the diffusion method and by determining the inhibition zone. The results showed that the oils had a great potential antibacterial activity against some bacteria. The maximum zone of inhibition was obtained against Enterococcus faecalis (22 mm). In contrast, the oils were ineffective on the inactivation of Lysteria monocytogenes and Escherichia coli. Antioxidant capacity was assessed by in vitro tests using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and significant activity was found for these A. arborescens oils.
This study was conducted to determine the antimicrobial (antibacterial and antifungal) activity of green oak (Quercus ilex) wood ash from Beni Snous region (Tlemcen, Algeria). For the evaluation of antimicrobial potency, we tested the effect of different ash concentrations on reference bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Enterobacter cloacae, and Proteus mirabilis) and reference yeasts (Candida albicans ATCC 10231 and Candida albicans 444IP). The antibacterial activity test results show that Gram-positive bacteria have a higher sensitivity than Gram-negative bacteria. Concerning the antifungal activity, inhibition of yeast growth was found even at low ash concentrations (5%). The results of this preliminary investigation thus revealed that wood ash from green oak exerts a remarkable antimicrobial activity.
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