Chrysanthemum (Chrysanthemum morifolium Ramat.) is a globally important ornamental plant with great economic, cultural, and symbolic value. However, research on chrysanthemum is challenging due to its complex genetic background. Here, we report a near-complete assembly and annotation for C. morifolium comprising 27 pseudochromosomes (8.15 Gb; scaffold N50 of 303.69 Mb). Comparative and evolutionary analyses reveal a whole-genome triplication (WGT) event shared by Chrysanthemum species approximately 6 million years ago (Mya) and the possible lineage-specific polyploidization of C. morifolium approximately 3 Mya. Multilevel evidence suggests that C. morifolium is likely a segmental allopolyploid. Furthermore, a combination of genomics and transcriptomics approaches demonstrate the C. morifolium genome can be used to identify genes underlying key ornamental traits. Phylogenetic analysis of CmCCD4a traces the flower colour breeding history of cultivated chrysanthemum. Genomic resources generated from this study could help to accelerate chrysanthemum genetic improvement.

Sassafras tzumu (Chinese sassafras) is an economically and ecologically important deciduous tree species. Over the past few decades, increasing market demands and unprecedented human activity in its natural habitat have created new threats to this species. Nonetheless, the distribution of its habitat and the crucial environmental parameters that determine the habitat suitability remain largely unclear. The present study modeled the current and future geographical distribution of S. tzumu by maximum entropy (MAXENT) and genetic algorithm for rule set prediction (GARP). The value of area under the receiver operating characteristic curve (AUC), Kappa, and true skill statistic (TSS) of MAXENT was significantly higher than that of GARP, indicating that MAXENT performed better. Temperate and subtropical regions of eastern China where the species had been recorded was suitable for growth of S. tzumu. Relative humidity (26.2% of permutation importance), average temperature during the driest quarter (16.6%), annual precipitation (12.6%), and mean diurnal temperature range (10.3%) were identified as the primary factors that accounted for the present distribution of S. tzumu in China. Under the climate change scenario, both algorithms predicted that range of suitable habitat will expand geographically to northwest. Our results may be adopted for guiding the preservation of S. tzumu through identifying the habitats susceptible to climate change.

YABBY (YAB) genes are specifically expressed in abaxial cells of lateral organs and determine abaxial cell fate. However, most studies have focused on few model plants, and the molecular mechanisms of YAB genes are not well understood. Here, we identified a YAB transcription factor in chrysanthemum (Chrysanthemum morifolium), Dwarf and Robust Plant (CmDRP), that belongs to a distinct FILAMENTOUS FLOWER (FlL)/YAB3 sub-clade lost in Brassicaceae. CmDRP was expressed in various tissues but did not show any polar distribution in chrysanthemum. Overexpression of CmDRP resulted in a semi-dwarf phenotype with a significantly decreased active GA3 content, while reduced expression generated the opposite phenotype. Furthermore, plant height of transgenic plants was partially rescued through the exogenous application of GA3 and Paclobutrazol, and expression of the GA biosynthesis gene CmGA3ox1 was significantly altered in transgenic plants. Yeast one-hybrid, LUC, and ChIP-qPCR analyses showed that CmDRP could directly bind to the CmGA3ox1 promoter and suppress its expression. Our research reveals a nonpolar expression pattern of a YAB family gene in dicots and demonstrates it regulates plant height through the GA pathway, which will deepen the understanding of the genetic and molecular mechanisms of YAB genes.