Entanglement harvesting from the quantum field is a well-known fact that, in recent times, is being rigorously investigated further in flat and different curved backgrounds. The usually understood formulation studies the possibility of two uncorrelated Unruh-DeWitt detectors getting entangled over time due to the effects of quantum vacuum fluctuations. Our current work presents a thorough formulation to realize the entanglement harvesting from non-vacuum background fluctuations. In particular, we further consider single excitation field states and a pair of inertial detectors, respectively, in (1 + 1) and (1 + 3) dimensions for this investigation. Our main observation asserts that entanglement harvesting is suppressed compared to the vacuum fluctuations in this situation. Our other observations confirm a non-zero individual detector transition probability in this background and vanishing entanglement harvesting for parallel co-moving detectors. We look into the characteristics of the harvested entanglement and discuss its dependence on different system parameters.
It is well-known that the (1 + 1) dimensional Schwarzschild and spatially flat FLRW spacetimes are conformally flat. This work examines entanglement harvesting from the conformal field vacuums in these spacetimes between two Unruh-DeWitt detectors, moving along outgoing null trajectories. In (1 + 1) dimensional Schwarzschild spacetime, we considered the Boulware and Unruh vacuums for our investigations. We also considered the momentum-space representation of Green's functions and linear couplings between the field and the detectors to estimate the concurrence and the mutual information corresponding to specific field mode frequency. In this analysis, one observes that while entanglement harvesting is possible in (1 + 1) dimensional Schwarzschild and (1 + 3) dimensional de Sitter spacetimes, it is not possible in the (1 + 1) dimensional de Sitter background for the same set of parameters when the detectors move along the same outgoing null trajectory. The qualitative results from the Boulware and the Unruh vacuums are alike. Furthermore, we observed that the concurrence depends on the distance d between the two null paths of the detectors in a periodic manner. In (1 + 1) dimensional Schwarzschild and (1 + 3) dimensional de Sitter backgrounds one obtains specific periodic points in d for which concurrence vanishes. While in (1 + 1) dimensional de Sitter spacetime, one gets specific periodic regions in d with vanishing concurrence. We also observe that the mutual information does not depend on d in (1 + 1) dimensional Schwarzschild and de Sitter spacetimes but periodically depends on it in (1 + 3) dimensional de Sitter background.
In recent years, the development of RNA-guided genome editing (CRISPR-Cas9 technology) has revolutionized plant genome editing. Under nutrient deficiency conditions, different transcription factors and regulatory gene networks work together to maintain nutrient homeostasis. Improvement in the use efficiency of nitrogen (N), phosphorus (P) and potassium (K) is essential to ensure sustainable yield with enhanced quality and tolerance to stresses. This review outlines potential targets suitable for genome editing for understanding and improving nutrient use (NtUE) efficiency and nutrient stress tolerance. The different genome editing strategies for employing crucial negative and positive regulators are also described. Negative regulators of nutrient signalling are the potential targets for genome editing, that may improve nutrient uptake and stress signalling under resource-poor conditions. The promoter engineering by CRISPR/dead (d) Cas9 (dCas9) cytosine and adenine base editing and prime editing is a successful strategy to generate precise changes. CRISPR/dCas9 system also offers the added advantage of exploiting transcriptional activators/repressors for overexpression of genes of interest in a targeted manner. CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) are variants of CRISPR in which a dCas9 dependent transcription activation or interference is achieved. dCas9-SunTag system can be employed to engineer targeted gene activation and DNA methylation in plants. The development of nutrient use efficient plants through CRISPR-Cas technology will enhance the pace of genetic improvement for nutrient stress tolerance of crops and improve the sustainability of agriculture.
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