Murashige and Skoog's medium with 2, 4-D and kinetin induced callus in the shoot segments of Asparagus racemosus. Regeneration of shoot buds and clonal multiplication of excised shoots through proliferation of nodal buds could be achieved by the use of IAA and BAP in the medium. Rooting was achieved with half strength MS basal medium plus IBA. Complete plants with cladode, crown and root systems were developed in hormone free medium. The plants were successfully transferred to soil.
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