The present study focused on the effect of Allium longicuspis extracts (ALe) against anthracnose of mango fruit. In vitro tests (mycelial growth and conidial germination) showed that, ALE concentrated from 0.75 to 2.5 g L −1 completely inhibited the growth of Colletotrichum gloesporioides. Cytoplasmic discharge, mycelial and conidial blasts were clearly observed when applied with ALE. The minimum effective concentration (MEC) of ALE at 0.75 g L 1 can be applied as protective, curative and simultaneous treatment in mango fruit to inhibit the anthracnose infection. Efficacy of garlic extract was relatively superior to synthetic fungicide based on protective, curative and simultaneous treatments. Twenty chemical components were detected in ALE based on GCMS analysis (Table 1). The six major components were the following: oleyl alcohol, methyl ether (42.04%), γ-sitosterol (15.85%), , 24-norursa-3.12-diene (5.62%), 1-octadecanol methyl ether (4.23%), n-pentadecanol (3.95%) and 2-vinyl-4h-1 3-dithiine (3.76%). The findings support the potential use of ALE as an alternative to synthetic fungicide. Mangifera indica L. popularly known as mango, is the second most important agricultural fruit in the Philippines with a production yield of 899.014 metric tons in 2016 1. Losses is as high as 2-33% due to anthracnose disease 2. Colletotrichum gloeosporioides (Penz.) Penz. and Sacc is a plant pathogenic fungi that dominantly attack mango fruit and main cause of anthracnose disease 3-7. The fungus is ubiquitous and responsible for many fruit diseases of other tropical fruit such as banana, avocado and many others 6-7. Furthermore, it infects the inflorescence, young leaves and branches, and its fruit 3,5. In the past, synthetic fungicides (dithiocarbamate, benomyl, thiabendazole, prochloraz, imazalil and copper fungicides) are used to control anthracnose infection 3,8,9. However, some of them are no longer used for mango export in other countries and is somehow restricted because of public concern over exceeding maximum residue limit (MRL) 3. Further, due to continuous used of synthetic fungicides, fungal pathogen developed resistance and it contaminates our environments 10. Therefore, the screening of potential fungicides derived from natural products (plants) may help to overcome this problem. Allium spp. on the other hand, has been widely utilized not only as spices for food but also for treatment against wide range of microorganisms including fungi 11-17. Organosulfur compounds are mainly produced by Allium spp. This compound penetrates the cell membrane and undergo thiol-disulphide exchange reactions in proteins 18. This information is mainly the basis of fungal death 19-20. Hence, organosulfur compound has multiple targets inside the cell and this fact can make it tough for the pathogen to develop resistance 18. Limited knowledge exists on the effect of Allium longicuspis or commonly known as "garlic: wild type" against C. gloeosporioides in vitro and in vivo tests. This may be useful in managing anthracnose incidence in m...