O corante pararosanilina (P) é um pigmento que apresenta um relevante papel na análise de substâncias química e bactérias do tipo Gram positivo e negativo, além de outros microorganismos. Os ensaios potenciométricos de P mostraram que o corante é um ácido monoprótico e fraco (pKa= 8,78); enquanto a espectrofotometria permitiu estimar seu coeficiente de absortividade molar (ε) igual a 1,48x10(4) mol-1 cm-1 L. A curva analítica para a determinação de P apresenta linearidade entre 7,36x10-6 a 7,36x10-5 mol L-1 e a metodologia foi aplicação em amostras biológicas.
Pararosaniline dye (P) is a pigment which has a significant role in analysis of chemical substances and gram-positive and negative bacteria, as well as other microorganisms. Potentiometric essays of P have shown that the colorant is a weak monoprotic acid (pKa= 8,78);and the spectrophotometry allows to estimate its molar absorption coefficient as 1,48x104 mol-1 cm-1 L. The analytical curve for a determination of P has linearity between 7,36x10-6 and 7,36x10-5 mol L-1, and the methodology was application in biological samples.
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