Kristiana R, Sibero MT, Farisa MY, Ayuningrum D, Dirgantara D, Hanafi M, Radjasa OK, Sabdono A, Trianto A. 2019. Antibacterial potential of nudibranch-associated bacteria from Saparua and Nusa Laut Islands, Indonesia. Biodiversitas 20: 1811-1819. Infections caused by multidrug-resistant bacteria are the international health issue that triggers the urgency of finding new antibacterial agents. The aim of this study was to obtain the nudibranchs-associated bacteria that have bioactivity against multidrug-resistant bacteria. A total of 13 species of nudibranch were identified based on morphological characterization. Overlay methods were used for the screening of the isolates bioactivity against six pathogenic multidrug-resistant bacteria. The Minimum Inhibition Concentration (MIC) of the crude extract was evaluated against Methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli, Bacillus subtilis, Klebsiella pneumonia, Micrococcus luteus, and Extended Spectrum Beta Lactamases E. coli (ESBL) using MTT method. A total of 145 isolates were obtained which eleven of the isolates showed antibacterial activity against the pathogenic bacteria. The MIC te sts showed that the best activity was isolate SM-S-9-15 and SM-N-3-7. The methanolic extract of isolate SM-S-9-15 active to all of the pathogenic bacteria, while the ethyl acetate extract of the isolate SM-N-3-7 active to the E. coli, B. subtilis, K. pneumonia at the concentration of 500 g/mL. According to 16S ribosomal RNA gene sequence-based identification, all active isolates belong to Virgibacillus marismortui, V. dokdonensis, Bacillus kochii, Vibrio algynoliticus, and Pseudoalteromonas piscicida.
Wijayanti DP, Sabdono A, Widyananto PA, Dirgantara D, Hidaka M. 2018. Bacterial symbionts of acroporid corals:Antipathogenic potency against Black Band Disease. Biodiversitas 19: 1236-1242. Black Band Disease (BBD), an infectious coraldisease which can cause a rapid decline of coral reefs, has appeared as a serious threat -to many reefs around the world includingKarimunjawa National Park, Java Sea. Although it had been studied for more than 30 years, control of disease remains obscure. In thepresent research coral symbiont bacteria having antipathogenic activity against Black Band bacterial associate were screened andcharacterized. Fourteen out of 87 bacteria isolates derived from healthy corals showed antagonism against the Black Band bacterialstrains. The isolates were then re-examined using disc-diffusion method to confirm the initial observation. The CI6 showed the strongestability to inhibit BAFBB5, a bacterial strain associated with the BBD. Following the partial sequencings of 16S rDNA, the resultsindicated that the CI6 isolate was closely related to Virgibacillus salarius strain SA-Vb1, while the BBD associate isolate has strongrelation with Virgibacillus marismortui. The results have shown that bacterial strains derived from healthy acroporid corals havepotential to be used as a biocontrol agent against BBD.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.