A thin-layer chromatographic method Is described for the detection of A9-tetrahydrocannabinol in blood and serum. The procedure utilizes a reagent, 2-p-chlorosulfophenyl-3-phenylindone, which reacts with the phenolic group to form a derivative. Following extraction and cleanup, the derivative Is prepared and separated from reagent and naturally occurring compounds by thin-layer chromatography. The derivative is then detected by spraying the thin-layer plate with an alkoxide spray which produces a fluorescent spot visible under long wave ultraviolet light. The method can detect 0.2 ng/ml of tetrahydrocannabinol in 5 ml of serum and is suitable for routine screening.
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