Abstract. The Hedgehog/Patched signaling pathway plays a prominent role during mammalian development but it is also involved in oncogenic transformation. We investigated the methylation status of the Patched promotor in a set of basocellular carcinomas of the skin and ovarian tumors as an alternative to mutational causes of the pathway deregulation. Our aim was to define a possible role of genetic and/or epigenetic mechanisms of Hedgehog/Patched signal transduction in the development of these tumors. Bisulfite-converted DNA from tumors and from matched healthy tissue was amplified by a specific PCR and the CpG-rich regions of the Patched promoter were sequenced. Two promoter regions showed statistically significant hypermethylation compared to healthy controls in ovarian tumors; more significantly in the region in the vicinity of Gli1-binding sites and less significantly in the region containing the ATG codon. But, in basocellular carcinomas of the skin we observed no difference in methylation, suggesting different mechanisms of neoplasia in these tumors.

The aim of this study was to assess the oral health condition of Croatian war veterans with post-traumatic stress disorder (PTSD). The study included 50 men who experienced combat stress and had been diagnosed with PTSD. They were compared with the group of 50 age-matched men who did not participate in war and did not have PTSD. Xerostomia, oral mucosal lesion, oral hygiene status, dental, and periodontal conditions, as well as temporomandibular disorders (TMDs) were assessed and compared between the groups. Subjects in PTSD group had poorer oral hygiene, and periodontal status compared with the control subjects. Furthermore, they had less teeth than the control group (P = 0.04). Although, there was no statistically significant difference between the groups in Decayed, Missing and Filled Teeth (DMFT) index (P = 0.36), PTSD group had more decayed and missing teeth, but less filled teeth (P < 0.05 for all). According the Research Diagnosis Criteria for Temporomandibular Disorder (RDC/TMD), PTSD patients had more TMD diagnoses compared with the control subjects. The most prevalent diagnosis was myofascial pain (48%) in PTSD group, while in the control group it was disc displacement with reduction (8%). It can be concluded that the oral health condition in PTSD patients is significantly affected compared with the control subjects.

The signaling pathway is activated in SCC and inducible in vitro by Shh protein.

Mental health outcomes of road traffic accidents (RTAs) are always investigated in assessments of those involved. The aim of this study was to investigate the psychological consequences and associated factors in all RTA survivors, irrelevant of their injury status. A cohort of 155 people was assessed one month after experiencing a RTA using self-reported measures for posttraumatic stress disorder (PTSD), depression, and anxiety. Associations between mental health outcomes and sociodemographic factors, pre-RTA health status, injury-related factors, and RTA details were analyzed. RTA survivors reported substantial rates of PTSD (32.3%) and depression (17.4%) symptoms, and low rates of anxiety (5.8%). Symptoms of depression were associated with below-average self-perceived economic status, irreligiousness, medication use, psychiatric medication use, and injury-related factors. PTSD symptoms were associated with female gender, below-average self-perceived economic status, previous psychiatric illness, medication use, psychiatric medication use, not being at fault in the relevant RTA, claiming compensation, and injury-related factors. Anxiety symptoms were associated with previous chronic or psychiatric illness, previous permanent pain, psychiatric medication use, and self-perceived threat to life, but not with sustaining injury. Along with the evaluation and treatment of RTA injuries, health care providers should evaluate the pre-RTA health status of all RTA victims. Psychological support to those at risk may prevent psychological disorders after RTAs.

Falling down was the most common cause of oro-maxillofacial injuries in both men and women and in all three age groups. The leading type of injury was bone injury. The data obtained from this study provide important information for future prevention from injuries.