The present research was carried out at Experimental Farm, Department of Agriculture, Mata Gujri College, Fatehgarh Sahib, Punjab during winter season of 2020-21. The experiment was laid out in randomized block design with seven treatments (Control, 20gy, 40gy, 60gy, 80gy, 100gy and 120gy) and the treatments were replicated thrice. From the experiment it can be concluded that the lower dose (20gy) of gamma irradiation show positive result on growth i.e. maximum plant height (90.03 cm), size of leave (28.44 cm) and take less number of days to sprout (12.65 days) and flowering of Gladiolus cultivar White Prosperity. As the dose of gamma irradiation increases (60gy-120gy), it affect the vegetative characteristic like days taken to sprouting, plant height, number of leaves, size and length of longest leaf, it reduces with the dose increase, but it has no effect on number of sprouts/corm and on size of corm.
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