Indoleamines are products of the pineal gland and are postulated to play an antigonadotrophic role in the reproductive system of mammals. In humans, indoleamines have been localized in tissue fluids such as plasma, serum and cerebrospinal fluid. Because indoleamines exhibit antigonadotrophic properties, the authors examined whether these agents cause inhibitory effects on sperm motility. In this study, time and dose-dependent inhibition of sperm motility by indoleamines was observed. Furthermore, the presence of indoles in incubation medium decreased sperm velocity. These data suggest that the presence of high doses of indoles in reproductive fluids may inhibit sperm motility and velocity.
Objective: The Philadelphia (Ph) chromosome, consisting of the t(9;22)(q34;q11) translocation, is observed in ~90% of patients with chronic myeloid leukemia (CML). Variant Ph translocations are observed in 5%-10% of CML patients. In variant translocations 3 and possibly more chromosomes are involved. Herein we report 6 CML patients with variant Ph translocations. Materials and Methods: Bone marrow samples were examined using conventional cytogenetic meth ods. Fluorescence in situ hybridization (FISH) with whole-chromosome paints and BCR-ABL 1D probes were used to confirm and/or complement the findings, and identify rearrangements beyond the resolution of conventional cytogenetic methods. Results: Variant Ph translocations in the 6 patients were as follows: t(7;22)(p22;q11), t(9;22;15) (q34;q11;q22), t(15;22)(p11;q11), t(1;9;22;3)(q24;q34;q11;q21), t(12;22)(p13;q11), and t(4;8;9;22) (q11;q13;q34;q11). Conclusion: Among the patients, 3 had simple and 3 had complex variant Ph translocations. Two of the presented cases had variant Ph chromosomes not previously described, 1 of which had a new complex Ph translocation involving chromosomes 1, 3, 9, 22, and t(1;9;22;3)(q24;q34;q11;q21) apart from a clone with a classical Ph, and the other case had variant Ph translocation with chromosomes 4, 8, 9, and 22, and t(4;8;9;22)(q11;q13;q34;q11) full complex translocation. Number of studies reported that some patients with variant Ph translocation were poor responders to imatinib. All of our patients with variant Ph translocations had suboptimal responses to imatinib, denoting a poor prognosis also. Variant Ph translocations may be important as they are associated with prognosis and therapy for CML patients. (Turk J Hematol 2011; 28: 186-92)
OBJECTIVES:Multiple genetic changes are observed in malignant tumors but are rare or absent in benign conditions. Aneuploidy is the most common feature of solid tumors including lung cancer and diagnosis of malignant tumors is possible through detection of aneuploidy. The aim of this study was to investigate chromosomal abnormalities in cells from non-small cell lung cancer patients obtained bronchoscopically and to evaluate the suitability of fluorescence in situ hybridization (FISH). MATERIAL AND METHODS:Bronchial lavage samples of 17 non-small cell lung cancer (NSCLC) patients were evaluated with fourcolor FISH using deoxyribonucleic acid (DNA) probes specific for the centromere regions of chromosomes 3, 7 and 8. tested specimens were first hybridized with probes, then visualized under fluorescence microscobeand captured with device's camera. RESULTS:High number of aneuploidic cells were detected in all the samples. Increased or decreased abnormal copies or chromosomes 3, 7 and 8 were obserced in all the 17 patients. Aneuploidy of chromosome 3 (21.35%) was higher than those of chromosome 7 (9.06%) and chromosome 8 (15.47%). Moreover, our results were significant for monosomy and trisomy of chromosome 3, trisomy of chromosome 7, nullisomy, monosomy and trisomy of, chromosome 8 (p< 0.05). CONCLUSION:It has been observed that FISH is a useful technique for detection of aneuploidy in bronchial lavage samples obtained by bronchoscopy. Interphase cells were evaluated without cell culturing with this method and high number of tumor cells were enumerated rapidly. Our study has demonstrated that, FISH technique may be used successfully in detection of chromosome number abnormalities in NSCLC patients and may facilitate evaluation of genetic abnormalities.
A AB BS S T TR RA AC CT T O Ob bj je ec ct ti iv ve e: : Recurrent pregnancy loss is an important problem affecting couples trying to conceive. Genetic factors, particularly chromosomal abnormalities appear to be highly associated with reproductive loss. The frequency of presence of at least one partner, who is a carrier of a structural chromosome rearrangement, varies from 3% to 11% among couples with a history of recurrent pregnancy loss. The aim of this study was to introduce the cytogenetic data of couples that referred with recurrent pregnancy loss to our center. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : Chromosome analyses were performed in 449 couples with more than one pregnancy loss using GTL banding. R Re es su ul lt ts s: : Chromosome abnormalities were detected in one partner in 19 of 449 couples. All chromosome abnormalities were structural, and 18 of them were balanced. Autosomal reciprocal translocations were the most frequent type (2.9%) of abnormalities. The unique Robertsonian translocation found in our study was t(13;14), which was observed in two patients. Chromosomal heteromorphisms were determined in 19.59% of patients. C Co on nc cl lu us si io on n: : The frequency of chromosomal abnormalities were found as 4.23% in our series. Cytogenetic investigation of couples with recurrent pregnancy loss is necessary as chromosomal abnormalities constitute a very important part of factors that cause pregnancy loss.K Ke ey y W Wo or rd ds s: : Cytogenetics; abortion, habitual; chromosomes Ö ÖZ ZE ET T A Am ma aç ç: : Tekrarlayan gebelik kayıpları, çocuk sahibi olmayı amaçlayan çiftleri etkileyen önemli bir sorundur. Genetik etkenler, özellikle de kromozom anomalilerinin üreme kayıplarıyla yakın ilişkisinin olduğu bilinmektedir. Tekrarlayan gebelik kaybı öyküsü olan çiftlerde, eşlerin en az birinin kromozom yapı anomalisi taşıyıcısı olma sıklığının %3-11 arasında değiştiği bildirilmektedir. Bu çalışmada, merkezimize tekrarlayan gebelik kayıpları nedeniyle başvuran çiftlerden elde edilen sitogenetik verilerin değerlendirilmesi amaçlanmıştır. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Birden fazla gebelik kaybı olan 449 çiftte G bantlama kullanılarak kromozom analizi gerçekleştirildi. B Bu ul lg gu ul la ar r: : 449 çiftin 19'unda eşlerden birinde kromozom anomalisi saptandı. Tüm kromozom anomalileri yapı anomalisi, 18'i ise dengeli olarak bulundu. En sık gözlenen kromozom anomalisi türü otozomal resiprokal translokasyonlar (%2.9) olarak bulundu. İki olguda saptanan t(13;14) bu çalışmada gözle-nen tek Robertson tipi translokasyon oldu. %19.59 oranında kromozomal heteromorfizm gözlendi. S So on nu uç ç: : Bu çalışmada, tekrarlayan gebelik kaybı öyküsü olan çiftlerde kromozom anomalilerinin sıklığı %4.23 olarak bulunmuştur. Kromozom anomalileri tekrarlayan gebelik kayıp sebeplerinin önemli bir bölümünü oluşturduğundan, tekrarlayan gebelik kayıpları olan çiftlerde sitogenetik inceleme yapılması gereklidir.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.