dBroad-range amplification and sequencing of the bacterial 16S rRNA gene directly from clinical specimens are offered as a diagnostic service in many laboratories. One major pitfall is primer cross-reactivity with human DNA which will result in mixed chromatograms. Mixed chromatograms will complicate subsequent sequence analysis and impede identification. In SYBR green real-time PCR assays, it can also affect crossing threshold values and consequently the status of a specimen as positive or negative. We evaluated two conventional primer pairs in common use and a new primer pair based on the dual priming oligonucleotide (DPO) principle. Cross-reactivity was observed when both conventional primer pairs were used, resulting in interpretation difficulties. No cross-reactivity was observed using the DPOs even in specimens with a high ratio of human to bacterial DNA. In addition to reducing cross-reactivity, the DPO principle also offers a high degree of flexibility in the design of primers and should be considered for any PCR assay intended for detection and identification of pathogens directly from human clinical specimens.
No previous studies in the literature have investigated the serial mediation impact of innovation capability and product innovation on the influence of market orientation on firm performance. The focus in this research is on the role of the combination of innovation capability and product innovation in the influence of market orientation on firm performance. To that end, it is aimed to examine the direct and indirect impacts of market orientation on firm performance (through innovation capability and product innovation, respectively). The data collected from 739 small and medium-sized enterprises (SMEs) in Turkey by employing the online survey method were tested using the SmartPLS 3 analysis programme. Pieces of empirical evidence have revealed that both market orientation and innovation capability act as important antecedents of product innovation. Moreover, mediation analysis demonstrates that innovation capability and product innovation in turn mediate the connection between market orientation and firm performance. Consequently, this research provides pieces of evidence showing that SMEs can increase their firm performance with innovations they can make in their products by improving their innovation capability with a market-oriented approach so that they can open up to foreign markets in theory and practice.
This study aims to develop a competing model to identify the best-fitting culture type in the relationship between organizational learning, management innovation, and New Product Development (NPD) performance, and it also aims to explore the moderator role of organizational culture in this relationship. The study collected data from 661 employees through survey methods from 234 firms in Turkey and tested with Structural Equation Modelling (SEM). The research shows that, despite the acceptable goodness of fit indices for each type in comparing four culture types, the market and adhocracy culture model is the best-fitting model and performs better with the best goodness of fit. In addition to the fact that the market and adhocracy cultures have partial moderator effects on the relationship between organizational learning, management innovation, and new product development, the study explains the relationship between organizational learning, new product development performance, and management innovation within a competing model based on organizational culture. It also provides a framework for firms to prioritize new product development to decide which cultural structure to choose.
The aim of this research is to examine the relationship between organizational culture, management innovation, product innovation and new product development performances. The data were collected through questionnaires on the production operations in Istanbul. The study first provides literature knowledge about organizational culture, management innovation, product innovation and new product development performance. In this context, research model and related hypotheses have been developed. In order to test the research hypotheses, a total of 594 managers of the 141 manufacturing companies operating in the manufacturing sector in Istanbul were used the data obtained by the questionnaire method. Data collected from 594 executives were analysed using SPSS software program. As a result of this study, it has been determined that product innovation has intermediary effects mediating on organizational culture dimensions and new product development performance. These empirical findings show that in the manufacturing sector, competitive advantage can be achieved through the development of businesses (in terms of organizational culture, management innovation and new product development performance relevance).
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